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Cytophotometric estimation of nuclear DNA content and karyotype analysis of eight cultivars of Trigonella foenum-graecum


Cytophotometric estimation of nuclear DNA content and karyotype analysis of eight cultivars of Trigonella foenum-graecum



Cytobios 91(366-367): 171-179



ISSN/ISBN: 0011-4529

The cytophotodensitometric estimation of 4C DNA content and karyotype analysis was carried out and the Somatic chromosome number of 2n = 16 was reported in eight cultivars of Trigonella foenum-graecum. Detailed karyotype analysis revealed cultivar specific chromosomal characteristics and minute structural alterations in chromosomes of the genome. The 4C DNA content also varied significantly from 9.950 to 12.940 pg. Highly significant variations in the genome length, volume and total form percentage were noted at cultivar level. Correlation coefficient studies revealed interdependence between the chromosomal and molecular parameters of the varieties. Variations in genomic structure and the nuclear DNA content despite the same somatic chromosome number suggest a genetic drift during microevolution leading to the development of new cultivars.

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Accession: 003084915

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