+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Cytotoxic T-lymphocyte-mediated lysis of Toxoplasma gondii-infected target cells does not lead to death of intracellular parasites



Cytotoxic T-lymphocyte-mediated lysis of Toxoplasma gondii-infected target cells does not lead to death of intracellular parasites



Infection and Immunity 66(10): 4651-4655



CD8+ T cells play a crucial role in the control of infection with intracellular microbes. The mechanisms underlying the CD8+ T-cell-mediated clearance of the intracellular pathogen Toxoplasma gondii are, however, not completely understood. The effect of CD8+ cytotoxic T-lymphocyte (CTL)-mediated lysis of host cells on the viability of intracellular T. gondii was investigated. Quantitative competitive PCR of the gene encoding T. gondii major surface antigen (SAG-1) was combined with treatment of the parasites with DNase, which removed the DNA template of nonviable parasites. The induction by CD8+ CTLs of apoptosis in cells infected with T. gondii did not result in the reduction of live parasites, indicating that intracellular T. gondii remains alive after lysis of host cells by CTLs.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003084986

Download citation: RISBibTeXText

PMID: 9746561


Related references

Cytotoxic T lymphocyte-mediated lysis via the Fc receptor of target cells. European Journal of Immunology 15(12): 1172-1177, 1985

Dna of nonhematopoietic target cells is fragmented upon cytotoxic t lymphocyte mediated lysis. Journal of Cellular Biochemistry Supplement (10 PART B): 83, 1986

Cloned human CD4-positive cytotoxic T lymphocytes specific for Toxoplasma gondii lyse tachyzoite-infected target cells. Journal of Immunology 151(4): 2024-2031, 1993

Free fatty acids inhibit cytotoxic T lymphocyte-mediated lysis of allogeneic target cells. Journal of Immunology 145(4): 1074-1077, 1990

Induced activation of the Toxoplasma gondii nucleoside triphosphate hydrolase leads to depletion of host cell ATP levels and rapid exit of intracellular parasites from infected cells. Journal of Biological Chemistry 273(20): 12352-9, 1998

Selective permeabilization of infected host cells with pore-forming proteins provides a novel tool to study protein synthesis and viability of the intracellular apicomplexan parasites Plasmodium falciparum and Toxoplasma gondii. Molecular and Biochemical Parasitology 112(1): 133-137, 2001

Target and effector cell fusion accounts for B lymphocyte-mediated lysis of mouse hepatitis virus-infected cells. Journal of General Virology 70: 1465-1472, 1989

Preferential activation and expansion of human peripheral blood gamma delta T cells in response to Toxoplasma gondii in vitro and their cytokine production and cytotoxic activity against T. gondii-infected cells. Journal of Clinical Investigation 96(1): 610-619, 1995

Lack of target cell participation in cytotoxic T lymphocyte-mediated lysis. Journal of Immunology 147(1): 55-59, 1991

Cross-reactive cytotoxic T-lymphocyte-mediated lysis of Chlamydia trachomatis- and Chlamydia psittaci-infected cells. Infection and Immunity 65(3): 951-956, 1997

Cytomegalovirus (CMV)-specific lysis of CMV-infected target cells can be mediated by both NK-like and virus-specific cytotoxic T lymphocytes. Clinical and Experimental Immunology 61(1): 80-89, 1985

Mechanism of cell-mediated cytotoxicity at the single cell level. VIII. Kinetics of lysis of target cells bound by more than one cytotoxic T lymphocyte. Journal of Immunology 132(5): 2190-2198, 1984

Intracellular reoviruses survive cytotoxic t lymphocyte mediated lysis of their host cell. Journal of Cellular Biochemistry Supplement (10 PART B): 90, 1986

Intracellular reovirus survives cytotoxic T lymphocyte-mediated lysis of its host cell. Journal of General Virology 68: 2899-2907, 1987

An in vitro study on lymphocyte-mediated immunity in mice experimentally infected with Toxoplasma gondii. Nihon Juigaku Zasshi. Japanese Journal of Veterinary Science 37(3): 235-243, 1975