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Derivation of efficient nisin producers by fusing protoplasts of two allied strains of Lactococcus lactis subsp. lactis with low nisin-synthesizing abilities



Derivation of efficient nisin producers by fusing protoplasts of two allied strains of Lactococcus lactis subsp. lactis with low nisin-synthesizing abilities



Microbiology New York 67(1): 38-44



The protoplasts of 2 allied strains, L. lactis subsp. lactis 729 and 1605, with low nisin-synthesizing abilities, were fused. Conditions promoting protoplast formation, fusion and regeneration were examined and the process was studied by electron microscopy. The effect of various selective agents in the regeneration medium on the segregation of recombinant clones was studied with respect to nisin production.

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Accession: 003087617

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