+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of antibodies to Candida albicans germ tubes during experimental infections by different Candida species



Detection of antibodies to Candida albicans germ tubes during experimental infections by different Candida species



Clinical and Diagnostic Laboratory Immunology 5(3): 369-374



Identification and characterization of Candida albicans germ tube-specific antigens may be of relevance for the serodiagnosis of invasive candidiasis since they could be the basis for the development of new diagnostic tests. In this study, we have identified two antigens of 180 and >200 kDa in the cell wall of C. albicans germ tubes which are responsible for the induction of antibodies to C. albicans germ tubes. Antigens of similar molecular masses have been demonstrated in the cell walls of the Candida species C. stellatoidea, C. parapsilosis, C. guilliermondii, C. tropicalis, and C. krusei, but not C. glabrata. The kinetics of the antibody responses to C. albicans germ tubes were studied in rabbits infected with different Candida species. Although these antibodies were detected in rabbits infected with all Candida species except C. glabrata, the kinetics of the antibody responses to C. albicans germ tubes induced by the Candida species studied were different. Both the highest titer and the earliest response of antibodies to C. albicans germ tubes were observed in rabbits infected with either of the two serotypes of C. albicans used. However, the time needed to elicit the antibodies to C. albicans germ tubes can be reduced as the result of an anamnestic antibody response. The results presented in this study show that a test designed to detect antibodies against C. albicans germ tube antigens may be suitable for the diagnosis of infections caused by most of the medically important Candida species.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003088875

Download citation: RISBibTeXText

PMID: 9605993


Related references

Simplified adsorption method for detection of antibodies to Candida albicans germ tubes. Journal of Clinical Microbiology 32(1): 217-219, 1994

Value of semiquantitative adsorption method for detection of antibodies to Candida albicans germ tubes. Mycoses 44(Suppl. 1): 11-12, 2001

Detection of antibodies to Candida albicans germ tubes in heroin addicts with systemic candidiasis. Clinical Microbiology and Infection 7(4): 224-226, 2001

Semi-quantitative adsorption for detection of antibodies to Candida albicans germ tubes by indirect immunofluorescence test. Mycoses 45(11/12): 461-464, 2002

Humoral antibodies against candida albicans a study of the diagnostic value of candida precipitins and agglutinins in candida colonization and candida infections with an without immunologic incompetence. Pediatric Research 7(1): 61, 1973

Detection of antibodies to Candida albicans germ tubes for diagnosis and therapeutic monitoring of invasive candidiasis in patients with hematologic malignancies. Journal of Clinical Microbiology 35(12): 3284-3287, 1997

Detection of antibodies to Candida albicans germ tube by immunofluorescence in immunosuppressed mice with experimental systemic candidiasis. 2007

Identification of antigens specific to germ-tubes of Candida albicans by monoclonal antibodies. Candida and candidamycosis: 223-226, 1991

Comparison of cream of rice agar and horse serum for differentiating germ tubes of Candida albicans from filaments of Candida tropicalis. Journal of Clinical Microbiology 5(4): 501-502, 1977

On the usefulness of various human sera for detection of germ tubes in candida albicans. Sowinski, Witold (Edited By) Proceedings Of The 2nd International Symposium on Medical Mycology October 6-8th, 1967, Poznan, Poland Xv+279p Illus Polish Dermatological Society: Warsaw, Poland 225, 1970

Rapid identification of Candida albicans and its related species Candida stellatoidea and Candida dubliniensis by a single PCR amplification using primers specific for the repetitive sequence (RPS) of Candida albicans. Journal of Dermatological Science 40(1): 43-50, 2005

Dispersion of germ tubes of Candida albicans in serum of experimental murine candidosis. Yeasts and yeast like microorganisms in medical science Proceedings of the Second International Specialized Symposium on Yeasts Tokyo 1972: 157-159, 1976

Experimental investigation of immunreactivity against Candida albicans. 1. Reaction of rabbits immunized with Candida albicans and challenged with living Candida albicans. Mykosen 14(8): 385-392, 1971

Rapid identification of Candida dubliniensis by indirect immunofluorescence based on differential localization of antigens on C. dubliniensis blastospores and Candida albicans germ tubes. Journal of Clinical Microbiology 36(9): 2428-2433, 1998

Candida albicans and non-C. albicans Candida species: comparison of biofilm production and metabolic activity in biofilms, and putative virulence properties of isolates from hospital environments and infections. Mycopathologia 175(3-4): 265-272, 2013