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Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay

Development and applications of a bovine coronavirus antigen detection enzyme-linked immunosorbent assay

Clinical and Diagnostic Laboratory Immunology 6(1): 130-132

A monoclonal antibody-based, antigen capture sandwich ELISA was developed for detection of bovine coronavirus. Faecal samples from calves with diarrhoea, experimentally infected calves, and a herd of adult dairy cattle with no signs of diarrhoea were tested. There was a close correlation between the results obtained with the ELISA and those obtained with electron microscopy and the haemagglutination test.

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Accession: 003091329

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PMID: 9874676

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