EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Development and validation of an improved enzyme-linked immunosorbent assay for the detection of thyroglobulin autoantibodies in canine serum samples



Development and validation of an improved enzyme-linked immunosorbent assay for the detection of thyroglobulin autoantibodies in canine serum samples



Domestic Animal Endocrinology 15(6): 525-536



An enzyme-linked immunosorbent assay to detect thyroglobulin autoantibodies (TGAB) in canine serum was developed and validated. The test result for each sample was derived from the optical density readings (OD) and expressed as an Ab-score(%) calculated from three in-house calibrators. The assay specifically detected TGAB as judged from lack of response in the assay after samples had been incubated with specific antigen. Intra- and interassay coefficients of variation ranged from 2.0-4.9% and 4.6-9.9%, respectively. The detection limit, an Ab-score of 5.6%, was close to the median Ab-score of 10% observed in healthy dogs (n = 132). The median Ab-score of dogs with primary hypothyroidism and lymphocytic thyroiditis (n = 11), skin diseases (n = 35), and non-thyroidal diseases (n = 63) was 340%, 12%, and 8%, respectively. The prevalence of TGAB in hypothyroid dogs with lymphocytic thyroiditis (sensitivity) was 91% (95% confidence limits: 59%-99%). In dogs with dermatological diseases without lymphocytic thyroiditis the prevalence of TGAB was 3% corresponding to a specificity of 97% (95% confidence limit: 85%-100%). In dogs with non-thyroidal diseases and healthy dogs the prevalence of TGAB was 5% and 6%, respectively. The diagnostic accuracy of serum TGAB was evaluated by subjecting the data from 11 dogs with lymphocytic thyroiditis and 35 control dogs without lymphocytic thyroiditis to receiver-operating characteristic curve analysis. The area under the receiver-operating characteristic curve (W = 0.966; 95% confidence limit 87%-100%) was significantly higher than that of a worthless test (0.5) (P < 0.0001), thereby indicating that serum TGAB measurements distinguished between dogs with and without lymphocytic thyroiditis.

(PDF emailed within 0-6 h: $19.90)

Accession: 003091630

Download citation: RISBibTeXText

PMID: 9861543

DOI: 10.1016/s0739-7240(98)00040-x



Related references

Development of an enzyme-linked immunosorbent assay for the detection of autoantibodies against thyroglobulin in chickens. Journal of Immunological Methods 69(2): 243-252, 1984

Prevalence of thyroglobulin autoantibodies detected by enzyme-linked immunosorbent assay of canine serum in hypothyroid, obese and healthy dogs in Japan. Research in Veterinary Science 76(2): 129-132, 2004

Prevalence of thyroglobulin autoantibodies detected by enzyme-linked immunosorbent assay of canine serum in hypothyroid, obese and healthy dogs in Japan. Research in Veterinary Science 76(2): 129-132, 2003

Development of an enzyme-linked immunosorbent assay for detection of circulating IgG autoantibodies against canine desmoglein 3 in dogs with pemphigus. Veterinary Dermatology 20(5-6): 331-337, 2010

Development and validation of an enzyme-linked immunosorbent assay for the measurement of canine pancreatic lipase immunoreactivity in serum. Journal of Veterinary Internal Medicine 15(3): 311, May-June, 2001

Development and analytic validation of an enzyme-linked immunosorbent assay for the measurement of canine pancreatic lipase immunoreactivity in serum. Canadian Journal of Veterinary Research 67(3): 175-182, 2003

Development and analytical validation of an enzyme linked immunosorbent assay for the measurement of canine gastric lipase immunoreactivity in serum. Canadian Journal of Veterinary Research 68(3): 161-168, 2004

Development and validation of an indirect Enzyme-linked immunosorbent assay for the detection of antibodies against Schmallenberg virus in blood samples from ruminants. Research in Veterinary Science 95(2): 731-735, 2014

Serodiagnosis of canine herpesvirus infection - development of an enzyme-linked immunosorbent assay and its comparison with two improved methods of serum neutralization test. Japanese Journal of Veterinary Science 52(2): 241-250, 1990

Validation of a commercially available enzyme-linked immunosorbent assay (ELISA) for the determination of cortisol in canine plasma samples. Veterinary Research Communications 22(3): 179-185, 1998

The detection of canine autoantibodies to thyroid antigens by enzyme-linked immunosorbent assay, hemagglutination and indirect immunofluorescence. Canadian Journal of Comparative Medicine 48(3): 262-267, 1984

Validation of 2 commercially available enzyme-linked immunosorbent assays for adiponectin determination in canine serum samples. Canadian Journal of Veterinary Research 74(4): 279-285, 2011

The detection of canine autoantibodies to thyroid antigens by enzyme linked immunosorbent assay elisa hemagglutination and indirect immunofluorescence. Canadian Journal of Comparative Medicine 48(3): 262-267, 1984

Enzyme-linked immunosorbent assay for determination of thyroglobulin autoantibodies. Comparison with RIA and haemagglutination. Acta Pathologica, Microbiologica, et Immunologica Scandinavica. Section C, Immunology 92(6): 377-382, 1984