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Development of an antigen spot test for detection of coronavirus in bovine fecal samples



Development of an antigen spot test for detection of coronavirus in bovine fecal samples



Clinical and Diagnostic Laboratory Immunology 6(4): 542-544



A rapid and sensitive micro-immunodot blot assay, the antigen spot test (AST), was developed for the detection of bovine coronavirus (BCV) antigen from neonatal calf faecal samples. The AST procedure can be completed in 3.5 h, whereas the previously reported immunodot blot assays require 10 to 12 h. 96 samples can be tested per membrane, and 10 membranes (960 samples) may be processed by a single technologist in one working day.

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Accession: 003092225

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PMID: 10391859


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