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Differentiation of four potyviruses using antibodies to intact or N- and C- or N-terminal peptide domains of coat proteins



Differentiation of four potyviruses using antibodies to intact or N- and C- or N-terminal peptide domains of coat proteins



International Journal of Tropical Plant Diseases 14(2): 157-166



Four commonly occurring potyviruses namely cowpea aphid borne mosaic (CABMV), eggplant mottle (EMoV), henbane mosaic (HMV) and potato Y (PVY) were purified and antisera produced. Antisera produced against native particles obtained 25 days after the start of immunization schedule showed more specificity in their reaction compared to antisera collected 35, 55 and 85 days after the start of immunization schedule. As the antisera collections were delayed more and more cross-reacting antibodies were produced. In contrast, antiserum obtained against SDS dissociated particles showed more or less similar kind of cross-reactions in all the four bleeds. Serological studies indicated that EMoV and PVY are more closely related to each other than to HMV, whereas CABMV showed fairly close these viruses was not possible due to the cross-reacting antibodies even in the first bleed antiserum of CABMV, EMoV and PVY. However affinity purified N-, N- and C-termini specific antibodies gave virus specific reaction in the case of CABMV and HMV while that of EMoV and PVY showed mutual cross-reactions indicating N-terminal epitope similarity between them.

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Accession: 003095998

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