Effect of dietary arachidonic acid on metabolism of deuterated linoleic acid by adult male subjects

Emken, E.A.; Adlof, R.O.; Duval, S.M.; Nelson, G.J.

Lipids 33(5): 471-480

1998


ISSN/ISBN: 0024-4201
PMID: 9625594
DOI: 10.1007/s11745-998-0230-x
Accession: 003106377

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Abstract
The influence of dietary supplementation with 20:4n-6 on uptake and turnover of deuterium-labeled linoleic acid (18:2n-6(d2)) in human plasma lipids and the synthesis of desaturated and elongated n-6 fatty acids from 18:2n-6(d2) were investigated in six adult male subjects. The subjects were fed either a high-arachidonic acid (HIAA) diet containing 1.7 g/d or a low-AA (LOAA) diet containing 0.21 g/d of AA for 50 d. Each subject was then dosed with about 3.5 g of 18:2n-6(d2) as the triglyceride (TG) at 8:00 A.M., 12:00, and 5:00 P.m. The total 18:2n-6(d2) fed to each subject was about 10.4 g and is approximately equal to one-half of the daily intake of 18:2n-6 in a typical U.S. male diet. Nine blood samples were drawn over a 96-h period. Methyl esters of plasma total lipid (TL), TG, phospholipid, and cholesterol ester were analyzed by gas chromatography-mass spectroscopy. Dietary 20:4n-6 supplementation did not affect uptake of 18:2n-6(d2) in plasma lipid classes over the 4-d study period nor the estimated half-life of 24-36 h for 18:2n-6(d2). The percentages of major deuterium-labeled desaturation and elongation products in plasma TL, as a percentage of total deuterated fatty acids, were 1.35 and 1.34% 18:3n-6(d2); 0.53 and 0.50% 20:2n-6(d2); 1.80 and 0.92% 20:3n-6(d2) and 3.13 and 1.51% 20:4n-6(d2) for the LOAA and HIAA diet groups, respectively. Trace amounts (
Effect of dietary arachidonic acid on metabolism of deuterated linoleic acid by adult male subjects