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Effect of steroid hormones on the secretion of cultured in vitro bovine oviductal epithelial cells and the influence of the secretory product on the development of pronuclear mouse embryos cultivated in vitro



Effect of steroid hormones on the secretion of cultured in vitro bovine oviductal epithelial cells and the influence of the secretory product on the development of pronuclear mouse embryos cultivated in vitro



Chinese Journal of Veterinary Science 17(4): 371-375



Bovine oviductal epithelial cells (BOEC) collected during dioestrus were cultured for two days in TCM-199 medium supplemented with estradiol-17- beta and progesterone, whose concentrations ranged from those found from day 0 (oestrus) to day 6 in bovine blood serum. After the secretory product of BOEC was separated by SDS-polyacrylamide gel electrophoresis, it was divided into two sizes of macromolecular proteins of 3.0x104-5.6x104-9.4x104, respectively.

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