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Effect of virulent and hypovirulent Cryphonectria parasitica (Murr.) Barr on the intercellular pathogen related proteins and on total protein pattern of chestnut (Castanea sativa Mill.)

Schafleitner, R.; Wilhelm, E.

Physiological and Molecular Plant Pathology 51(5): 323-332

1997


ISSN/ISBN: 0885-5765
DOI: 10.1006/pmpp.1997.0118
Accession: 003114759

Chestnut blight disease is caused by virulent strains of Cryphonectria parasitica. Strains infected with virus have reduced virulence, i.e. are hypovirulent. Chestnut stems were inoculated with virulent and hypovirulent strains and proteins recovered from the intercellular fluid of both stems and leaves. Infection with a hypovirulent strain resulted in induction of more extracellular pathogen-related proteins (beta-1,3-glucanase and chitinase), locally and systemically, than infection with a virulent strain. Seven days after inoculation with C. parasitica, two-dimensional gel electrophoresis revealed five proteins in total extracts of stems inoculated with virulent C. parasitica, and six other proteins in extracts from stems infected with hypovirulent C. parasitica. These results indicate that, besides the decreased virulence of the hypovirulent fungus, recognition and induction of defence mechanisms by the plant could be responsible for the survival of chestnut trees infected with hypovirulent strains.

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