Elicitation of thiophene production by cultured hairy roots of Tagetes patula

Rajasekaran, T.; Madhusudan, R.; Ravishankar, G.A.

Acta Physiologiae Plantarum 21(3): 243-247

1999


ISSN/ISBN: 0137-5881
DOI: 10.1007/s11738-999-0038-4
Accession: 003124265

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Abstract
Hairy roots of T. patula were initiated by infecting seedlings with Agrobacterium rhizogenes. The excised hairy roots were cultured in liquid Murashige and Skoog medium supplemented with sucrose, and were treated with elicitors obtained from mycelial cultures of Aspergillus niger, Rhizopus oligosporus and Penicillium notatum. The HPLC separation profile of the thiophenes indicated the presence of several structurally different thiophenes, alpha -terthienyl being predominant. Greatest production of thiophenes was recorded at the end of the fourth week in culture. Treatment of hairy roots with a mycelial extract of A. niger elicited an increase in thiophene of 1.6-fold over the control.

Elicitation of thiophene production by cultured hairy roots of Tagetes patula