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Enzymatic spectrophotometric determination of nitrites in beer



Enzymatic spectrophotometric determination of nitrites in beer



Analytical Letters 32(11): 2217-2227



A colorimetric assay for the estimation of nitrite in beer based on c-type multiheme enzyme nitrite reductase (NiR) isolated from Desulfovibrio desulfuricans ATCC 27774, was developed. Using the enzyme in solution, nitrite assay was linear in the 10-8-10-2 M range with a detection limit of 10-8 M and a recovery in the range 90-107%. The imprecision ranged from 4 to 10% on the entire calibration curve.

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Accession: 003127058

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DOI: 10.1080/00032719908542965



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