EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Enzyme-linked immunosorbent assay for the detection of Cryptosporidium parvum IgG in the serum of cats



Enzyme-linked immunosorbent assay for the detection of Cryptosporidium parvum IgG in the serum of cats



Journal of Parasitology 83(5): 957-960



The objective was to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of Cryptosporidium parvum IgG in the serum of cats. The ELISA was an indirect ELISA using soluble C. parvum oocyst antigens and a peroxidase-labeled anti-feline IgG secondary antibody. Sera from cats with Toxocara felis, Giardia spp., Aelurostrongylus abstrusus, Isospora felis, Isospora rivolta, Toxoplasma gondii, or Taenia spp. infections were assayed in specificity studies. Following optimization, the ELISA and fecal examination for oocysts were performed on samples from 170 client-owned or humane society source cats and 1 cat inoculated orally with C. parvum oocysts. Cryptosporidium parvum oocysts were detected in feces (4/170; 2.4%), and C. parvum IgG was detected in serum (26/170; 15,3%) from naturally exposed cats. The seroprevalence data suggest that some cats in the geographical area studied were exposed to C. parvum, but persistent oocyst shedding was less common. The ELISA is not useful for predicting oocyst shedding in individual cats.

(PDF same-day service: $19.90)

Accession: 003127154

Download citation: RISBibTeXText

PMID: 9379309

DOI: 10.2307/3284299



Related references

Development and evaluation of an enzyme-linked immunosorbent assay using recombinant p23 for the detection of antibodies to Cryptosporidium parvum in cattle. Journal of Protozoology Research 16(1-2): 9-15, 2006

Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 28(12): 2770-2774, 1990

Detection of Cryptosporidium parvum antigen in different specimens by co-agglutination and enzyme immunosorbent assay. Acta Parasitologica 45(3): 201, July, 2000

Enzyme-linked immunosorbent assay for the detection of circulating antigens of Toxoplasma gondii in the serum of cats. American Journal of Veterinary Research 50(9): 1586-1590, 1989

Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23. Onderstepoort Journal of Veterinary Research 76(2): 161-165, 2010

Immobilized enzyme-linked DNA-hybridization assay with electrochemical detection for Cryptosporidium parvum hsp70 mRNA. Analytical Chemistry 75(15): 3890-3897, 2003

Evaluation of an enzyme-linked immunosorbent assay for detection of Cryptosporidium spp. in stool specimens. Journal of Clinical Microbiology 31(6): 1468-1471, 1993

Evaluation of an antigen capture enzyme-linked immunosorbent assay for detection of Cryptosporidium oocysts. Journal of Clinical Microbiology 31(8): 2080-2084, 1993

Comparison of anti-human insulin antibodies detection by commercial enzyme-linked immunosorbent assay kit, displacement enzyme-linked immunosorbent assay and radioimmunoassay, in Thai diabetic patients. Diabetes Research & Clinical Practice. 22(1): 71-82, 1993

Interlaboratory evaluation of indirect enzyme-linked immunosorbent assay, antibody capture enzyme-linked immunosorbent assay, and immunoblotting for detection of immunoglobulin M antibodies to Toxoplasma gondii. Journal of Clinical Microbiology 25(1): 100-105, 1987

Enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin M and A antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 17(6): 997-1004, 1983

Cryptosporidium-specific antigen detection by enzyme-linked immunosorbent assay in preserved stool specimens. Abstracts of the General Meeting of the American Society for Microbiology 97(0): 203, 1997

Detection of Cryptosporidium specific antigen by enzyme-linked immunosorbent assay in preserved stool specimens. Abstracts of the General Meeting of the American Society for Microbiology 93(0): 521, 1993

Re-evaluation of commercially available enzyme-linked immunosorbent assay for the detection of Giardia lamblia and Cryptosporidium spp from stool specimens. Southeast Asian Journal of Tropical Medicine and Public Health 36 Suppl 4: 26-29, 2006