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Equine arteritis virus derived from an infectious cDNA clone is attenuated and genetically stable in infected stallions






Virology 260(1): 201-208

Equine arteritis virus derived from an infectious cDNA clone is attenuated and genetically stable in infected stallions

Two stallions inoculated intranasally with equine arteritis virus (EAV030H), derive from an infectious cDNA clone, did not develop clinical signs of the disease.

Accession: 003127886

PMID: 10405372

DOI: 10.1006/viro.1999.9817

Download PDF Full Text: Equine arteritis virus derived from an infectious cDNA clone is attenuated and genetically stable in infected stallions



Related references

Boon, J. den; Snijder, E.; Chirnside, E.; Vries, T. de; Horzinek, M.; Spaan, W., 1992: Genetic manipulation of equine arteritis virus: towards an infectious cDNA clone. A genomic cDNA library of the Bucyrus strain of equine arteritis virus has been constructed and the nucleotide sequence determined [den Boon et al (1991) Journal of Virology 65 2910-2920]. The genome encodes 6 open reading frames (ORFs 2 to 7) loc...

Zhang, J.; Go, Y.Young.; Huang, C.M.; Meade, B.J.; Lu, Z.; Snijder, E.J.; Timoney, P.J.; Balasuriya, U.B.R., 2012: Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteritis virus. A stable full-length cDNA clone of the modified live virus (MLV) vaccine strain of equine arteritis virus (EAV) was developed. RNA transcripts generated from this plasmid (pEAVrMLV) were infectious upon transfection into mammalian cells, and the r...

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Balasuriya, U.B.R.; Dobbe, J.C.; Heidner, H.W.; Smalley, V.L.; Navarrette, A.; Snijder, E.J.; Maclachlan, N.J.mes, 2004: Characterization of the neutralization deten-ninants of equine arteritis virus using recombinant chimeric viruses and site-specific mutagenesis of an infectious cDNA clone. We have used an infectious cDNA clone of equine arteritis virus (EAV) and reverse genetics technology to further characterize the neutralization determinants in the GP5 envelope glycoprotein of the virus. We generated a panel of 20 recombinant vir...

Mondal, S.P.; Cook, R.Frank.; Chelvarajan, R.Lakshman.; Henney, P.J.; Timoney, P.J.; Balasuriya, U.B.R., 2016: Development and characterization of a synthetic infectious cDNA clone of the virulent Bucyrus strain of equine arteritis virus expressing mCherry (red fluorescent protein). Strains of equine arteritis virus (EAV) differ in their virulence phenotypes, causing anywhere from subclinical infections to severe disease in horses. Here, we describe the in silico design and de novo synthesis of a full-length infectious cDNA c...

Golnik, W.; Cierpisz, J., 1994: Reproductive use of stallions naturally infected with equine arteritis virus. The investigations were carried out in 92 stallions in a stud where 83.2% of animals were negative for antibodies to equine arteritis virus (EAV). Of 3 seropositive stallions initially selected for test breeding, one had EAV in semen as demonstrat...

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Little, T.V.; Holyoak, G.R.; McCollum, W.H.; Timoney, P.J., 1992: Output of equine arteritis virus from persistently infected stallions is testosterone-dependent. Several unpublished observations suggest that persistence of equine arteritis virus (EAV) in the accessory sex glands of the stallion is dependent upon testosterone. To confirm this dependence, 15 stallions infected naturally with EAV for a minimu...