EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Equine infectious anemia virus transactivator is a homeodomain-type protein


Journal of Molecular Biology 277(4): 749-755
Equine infectious anemia virus transactivator is a homeodomain-type protein
Lentiviral transactivator (Tat) proteins are essential for viral replication. Tat proteins of human immunodeficiency virus type 1 and bovine immunodeficiency virus form complexes with their respective RNA targets (Tat responsive element, TAR), and specific binding of the equine anemia virus (EIAV) Tat protein to a target TAR RNA is suggested by mutational analysis of the TAR RNA. Structural data on equine infectious anemia virus Tat protein reveal a helix-loop-helix-turn-helix limit structure very similar to homeobox domains that are known to bind specifically to DNA. Here we report results of gel-shift and footprinting analysis as well as fluorescence and nuclear magnetic resonance spectroscopy experiments that clearly show that EIAV Tat protein binds to DNA specifically at the long terminal repeat Pu.1 (GTTCCTGTTTT) and AP-1 (TGACGCG) sites, and thus suggest a common mechanism for the action of some of the known lentiviral Tat proteins via the AP-1 initiator site. Complex formation with DNA induces specific shifts of the proton NMR resonances originating from amino acids in the core and basic domains of the protein.

Accession: 003127921

PMID: 9545368

DOI: 10.1006/jmbi.1998.1636

Download PDF Full Text: Equine infectious anemia virus transactivator is a homeodomain-type protein



Related references

The solution structure of the transactivator protein of the equine infectious anemia virus. Biological Chemistry Hoppe-Seyler 375(SPEC SUPPL 1): S111, 1994

Structural investigations of the transactivator-protein of the equine infectious anemia virus and its complex to RNA by NMR. Biological Chemistry Hoppe-Seyler 374(9): 685, 1993

Direct propagation of a wild type equine infectious anemia virus from serum of a horse with acute equine infectious anemia in equine dermis cells. Abstracts of the Annual Meeting of the American Society for Microbiology 86: 324, 1986

Detection of equine infectious anemia virus in horse leukocyte cultures derived from horses in various stages of equine infectious anemia viral infection. American Journal of Veterinary Research 45(1): 20-25, 1984

Experimental studies on equine infectious anemia (EIA). IV. Alteration of blood in mouse inoculated with the mouse-fixed equine infectious anemia virus and re-transmission test of the virus to a pony. Japanese Journal of Microbiology 4: 97-103, 1960

Equine tetherin blocks retrovirus release and its activity is antagonized by equine infectious anemia virus envelope protein. Journal of Virology 88(2): 1259-1270, 2014

Lymphadenopathy associated virus human t lymphotropic virus type iii gag gene product p 24 shares antigenic determinants with equine infectious anemia virus but not with visna virus or caprine arthritis encephalitis virus. Journal of Cellular Biochemistry Supplement (10 PART A): 189, 1986

Binding of equine infectious anemia virus to the equine lentivirus receptor-1 is mediated by complex discontinuous sequences in the viral envelope gp90 protein. Journal of General Virology 89(Pt 8): 2011-2019, 2008

Presentation and binding affinity of equine infectious anemia virus CTL envelope and matrix protein epitopes by an expressed equine classical MHC class I molecule. Journal of Immunology 171(4): 1984-1993, 2003

Physicochemical studies of equine infectious anemia virus. IV. Determination of the nucleic acid type in the virus. Archiv für die Gesamte Virusforschung 31(3): 273-280, 1970