Establishment of a callus culture system of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii x Populus plantierensis

Shen HaiLong; Watanabe, S.; Ide, Y.

Bulletin of the Tokyo University Forests 99: 19-26

1998


Accession: 003128577

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Abstract
A basic protocol was established for the callus culture of Populus euphratica, Populus alba cv. 'Pyramidalis' and Populus maximowiczii x Populus plantierensis , NAA and 2,4-D in combination or alone. The callus could be subcultured on MS medium supplemented with 0.2 mg/litre BAP and 1.0 mg/litre NAA. When callus was cultured on the medium containing BAP in combination with NAA or GA3, adventitious shoots were regenerated. The shoots were successfully rooted on 1/2 MS medium. Callus culture and plant regeneration were possible in all species tested. However, there were some differences in the hormonal condition suitable for callus formation and shoot regeneration among species.