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Establishment of a callus culture system of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii x Populus plantierensis


Establishment of a callus culture system of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii x Populus plantierensis



Bulletin of the Tokyo University Forests 99: 19-26



A basic protocol was established for the callus culture of Populus euphratica, Populus alba 'Pyramidalis' and Populus maximowiczii x Populus plantierensis ('FS-51'). Callus was induced from leaf explants on MS medium containing BAP NAA and 2,4-D in combination or alone. The callus could be subcultured on MS medium supplemented with 0.2 mg/litre BAP and 1.0 mg/litre NAA. When callus was cultured on the medium containing BAP in combination with NAA or GA3, adventitious shoots were regenerated.

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