+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Evaluation of serum neutralizing antibodies to bovine coronavirus in cows and their calves using Hmlu-1 cells



Evaluation of serum neutralizing antibodies to bovine coronavirus in cows and their calves using Hmlu-1 cells



Indian Journal of Medical Research 108(JULY): 8-11



Between 1992 and 1993, 75 paired serum samples from Holstein dairy cows and their calves were collected from Aomori, Tochigi and Okiniwa Prefectures, and the neutralizing antibody titres to bovine coronavirus (BCV) were determined using hamster lung (Hmlu)-1 cells. The anti-BCV antibody positive rate in the maternal serum samples was significantly lower (P < 0.001) in Okinawa (72%) than in Aomori (100%) or Tochigi (100%). The geometric mean titre (GMT) of anti-BCV neutralizing antibody was also significantly lower (P < 0.05) in maternal sera from Okinawa (89) than that of Aomori (229) or Tochigi (264). The anti-BCV neutralizing antibody titres in the sera of calves which had ingested the colostrum, significantly correlated with the antibody concentration of the maternal serum samples (P < 0.05). These results suggest an extensive BCV infection among the dairy cattle in these prefectures, with a varied pattern of distribution between the prefectures. Anti-BCV neutralizing antibody in the sera of newborn calves appeared to be transferred from their dams through colostrum.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003132831

Download citation: RISBibTeXText

PMID: 9745212


Related references

Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot. American Journal of Veterinary Research 78(9): 1065-1076, 2017

Isolation of bovine coronavirus (BCoV) in monolayers of HmLu-1 cells. Brazilian Journal of Microbiology 36(3): 207-210, 2005

Kinetics of neutralizing antibodies against bovine diarrhoea virus strains in calves after intake of colostrum from serologically BVD-positive cows. II. 34-70 weeks old. Kinetik neutralisierender Antikorper gegen BVD Virusstamme bei Kalbern nach Aufnahme von Kolostrum serologisch BVD positiver Mutter Beitrag II: 34 bis 70 Lebenswoche: 40, 1978

Development of nasal, fecal and serum isotype-specific antibodies in calves challenged with bovine coronavirus or rotavirus. Veterinary Immunology and Immunopathology 17(1-4): 425-439, 1987

Effect of serum and nasal neutralizing antibodies on bovine respiratory syncytial virus infection in calves. Journal of Infectious Diseases 134(4): 409-413, 1976

Specific colostral immunity in calves of cows vaccinated against foot-and-mouth disease. I. Demonstration of serum-neutralizing antibodies. Vet. Med, Praha 11: 303-310, 1966

Presence of neutralizing antibodies to bovine infectious rhinotracheitis virus in the serum of dairy cows. Atti Soc. ital, Buiatna, Piacenza 1: 111-115, 1969

Kinetics of neutralizing antibodies against bovine diarrhoea virus strains in calves after intake of colostrum from cows serologically positive for the virus. I. One to 32 weeks old. Kinetik neutralisierender Antikorper gegen BVD Virusstamme bei Kalbern nach Aufnahme von Kolostrum serologisch BVD positiver Mutter Beitrag I: Erste bis 32 Lebenswoche: 37, 1977

Neutralisation antibodies for bovine herpes virus 1 blood serum of pregnant cows and calves and in colostrum. Veterinarski Glasnik 45(11-12): 791-795, 1991

Detection of serum neutralizing antibodies against infectious bovine rhinotracheitis and infectious pustular vulvovaginitis in dairy cows. 1978

Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161(2): 410-420, 1987

Monoclonal antibodies to bovine coronavirus glycoproteins E2 and E3: demonstration of in vivo virus-neutralizing activity. Journal of General Virology 70: 993-998, 1989

Immunization against bovine rotavirus and coronavirus in pregnant cows. II. Antibody titres in the mammary secretion of vaccinated animals. III. Antibody titres in the blood serum of the calves. Medicina Veterinaria 8(4;5): 217...224,289...298, 1991

Isolation and characterization of bovine coronavirus from calves and adult cows with diarrhea. RDA Journal of Veterinary Science 39(2): 11-18, 1997

Neutralizing patterns of anti-bovine rotavirus (Lincoln) serum against cytopathic bovine rotaviruses isolated from calves in Japan. Microbiology and Immunology 25(10): 1097-1100, 1981