+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR

Experimental inoculation of adult dairy cows with bovine coronavirus and detection of coronavirus in feces by RT-PCR

Archives of Virology 144(1): 167-175

A reverse transcriptase PCR (RT-PCR) targeting a 407 bp fragment of the nucleocapsid gene of bovine coronavirus (BCV) was developed for detection of BCV RNA in feces of experimentally inoculated cattle. The sensitivity and specificity of the RT-PCR were confirmed using tissue culture-adapted BCV strains and feces of 2 calves inoculated with BCV. Ten nonpregnant, BCV seropositive, adult dairy cows were inoculated with winter dysentery (WD) (n = 8) or calf diarrhea (CD) (n = 2) strains of BCV intranasally and orally (n = 2) or through a surgically-placed duodenal catheter (n = 8) with and without dexamethasone treatment or feeding ice water. The 6 cows inoculated with BCV intranasally and through a duodenal catheter (2 of 2 cows given CD BCV and 4 of 6 cows given WD BCV) developed mild diarrhea, and BCV was detected in diarrheal feces by RT-PCR, ELISA or immune electron microscopy. These results suggest that CD and WD strains of BCV can cause diarrhea in adult cows in conjunction with host or environmental factors and that RT-PCR might be useful to diagnose BCV infections in calves and adult cows.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003135837

Download citation: RISBibTeXText

PMID: 10076517

DOI: 10.1007/s007050050493

Related references

Outbreak of diarrhea in cows from a dairy herd in the southern region of Minas Gerais State: detection of bovine coronavirus in the feces. Arquivo Brasileiro de Medicina Veterinaria E Zootecnia 61(4): 992-995, 2009

Adult dairy cow inoculation studies of bovine coronavirus. Special circular (145) 63-65, 1994

Bovine coronavirus detection in adult cows in Brazil. Arquivos do Instituto Biologico Sao Pauloabr-jun; 69(2): 103-104, 2002

Genomic relationship of porcine hemagglutinating encephalomyelitis virus to bovine coronavirus and human coronavirus OC43 as studied by the use of bovine coronavirus S gene-specific probes. Archives of virology40(7): 1215-1223, 1995

Antigenic relationships among proteins of bovine coronavirus, human respiratory coronavirus OC43, and mouse hepatitis coronavirus A59. Journal of Virology 51(2): 384-388, 1984

Detection of bovine coronavirus in feces by reversed passive hemagglutination. Archives of Virology 80(1): 23-31, 1984

Specific real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirus. Journal of Virological Methods 163(2): 452-458, 2010

Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction. Journal of Virological Methods 131(1): 86-91, 2006

Incidence of diarrhoea and of rotavirus- and coronavirus-shedding in calves, whose dams had been vaccinated with an experimental oil-adjuvanted vaccine containing bovine rotavirus and bovine coronavirus. Journal of Veterinary Medicine, B Infectious Diseases, Immunology, Food Hygiene, Veterinary Public Health 35(3): 186-196, 1988

Winter dysentery in cows associated with Bovine Coronavirus (BCoV) FT Disenteria de inverno associada ao coronavirus bovino (BCoV) em vacas. 2007

First detection of bovine noroviruses and detection of bovine coronavirus in Australian dairy cattle. Australian Veterinary Journal 96(6): 203-208, 2018

Characterization of monoclonal and polyclonal antibodies to bovine enteric coronavirus: establishment of an efficient ELISA for antigen detection in feces. Veterinary Microbiology 20(2): 111-122, 1989

Isolation and characterization of bovine coronavirus from calves and adult cows with diarrhea. RDA Journal of Veterinary Science 39(2): 11-18, 1997

Detection of Bovine Coronavirus in Healthy and Diarrheic Dairy Calves. Journal of Veterinary Internal Medicine 31(6): 1884-1891, 2017

Development of monoclonal antibody ELISA for simultaneous detection of bovine coronavirus, rotavirus serogroup A, and Escherichia coli K99 antigen in feces of calves. American Journal of Veterinary Research 53(1): 36-43, 1992