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Expression of myosin heavy chain isoforms in porcine muscles determined by multiplex PCR

Expression of myosin heavy chain isoforms in porcine muscles determined by multiplex PCR

Journal of food science 64(2): 222-225

A cDNA sequence of 5'-terminal region of myosin heavy chain (MHC) isoform 2x was determined. Based on this and published sequences, the procedure of reverse transcription-polymerase chain reaction (RT-PCR) employing a multiplex PCR technique was developed to analyze the expression patterns of MHC isoforms in porcine muscles. All four MHC isoforms in postnatal porcine muscles could be analyzed by this method and showed different expression patterns according to the porcine muscle types. The procedure developed in this study would be very useful to elucidate the relationships between meat quality and MHC isoform composition.

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Accession: 003136896

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DOI: 10.1111/j.1365-2621.1999.tb15869.x

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