Factors affecting the induction of somatic embryogenesis from apomictic apple embryos

U.R.hman, H.; James, D.J.; Caligari, P.D.S.; Wetten, A.

Tree biotechnology towards the millennium: 101-113

1998


Accession: 003138036

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Abstract
The potential for somatic embryogenesis was assessed in cotyledon cultures of 4 apomictic Malus species, viz. M. hupehensis, M. toringoides, M. sikkimensis and M. sargentii [M. toringo]. Eighty-day-old apomictic embryos were found to have the highest potential (43%). Induction of somatic embryos was investigated using 4 culture media comparing different concentrations of sucrose and sorbitol (0 or 60 g/litre) and oxoid agar (7 mg/litre) or Gelrite (2.5 mg/litre). Sucrose at 60 g/litre and gelrite favoured somatic embryogenesis. The frequency of somatic embryogenesis did not differ between species. The formation of adventitious roots, shoots and callus was also associated with somatic embryogenesis. Conversion of the embryoids into plantlets was achieved after 60 days of cold treatment on half-strength MS medium. Clonal fidelity of the individual clones was assessed using random amplified polymorphic DNA (RAPD) molecular markers. Somatic embryos from cotyledons lacked clonal fidelity. M. toringoides exhibited more genetic variation than M. hupehensis.