+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Fertile transgenic indica rice produced by expression of maize ubiquitin promoter-bar chimaeric gene in the protoplasts



Fertile transgenic indica rice produced by expression of maize ubiquitin promoter-bar chimaeric gene in the protoplasts



Journal of Plant Biochemistry & Biotechnology 6(2): 69-73



We report production of fertile transgenic Indica rice plants by transferring a chimeric construct consisting of promoter, first exon and intron of maize ubiquitin gene (Ubi-1) and the coding sequences of the bar gone from Streptomyces hygroscopicus to the rice protoplasts through electroporation. In total, 11 plants were regenerated. All of them were fertile and set seeds on maturity. These plants were resistant to high concentration of PPT (400 mg l-1) which was otherwise toxic to the untransformed controls. The gene was inherited to the progenies of the five plants in Mendelian ratio.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003140509

Download citation: RISBibTeXText

DOI: 10.1007/bf03263013


Related references

Expression of a Maize Ubiquitin Gene Promoter-bar Chimeric Gene in Transgenic Rice Plants. Plant Physiology 100(3): 1503-1507, 1992

Expression of a Maize Ubiquitin Gene Promoter-Bar Chimeric Gene in Transgenic Rice Plants. Plant Physiology 100(3): 1503-1507, 1992

Transgenic fertile indica rice plants regenerated from protoplasts. Journal of Cellular Biochemistry Suppl. (14 Part E): 279, 1990

Marker gene expression driven by the maize ubiquitin promoter in transgenic wheat. Annals of Applied Biology 136(2): 167-172, 2000

Activity of a maize ubiquitin promoter in transgenic rice. Plant molecular biology 23(3): 567-581, 1993

Activity of a maize ubiquitin promoter in transgenic rice. Plant Physiology 99(1 Suppl.): 19, 1992

Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants. Shi Yan Sheng Wu Xue Bao 35(4): 296-302, 2002

Heritable transgene expression pattern imposed onto maize ubiquitin promoter by maize adh-1 matrix attachment regions: tissue and developmental specificity in maize transgenic plants. Plant Cell Reports 22(12): 931-938, 2004

Promoter Elements Required for Developmental Expression of the Maize Adh1 Gene in Transgenic Rice. The Plant Cell 6(6): 799-810, 1994

Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice. Plant Cell 6(6): 799-810, 1994

Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice. Plant Science (Shannon) 124(1): 49-56, 1997

Transgenic indica rice (Oryza sativa L.) plants obtained by direct gene transfer to protoplasts. Journal of Biotechnology 32(1): 1-10, 1994

Pollen-specific expression of Oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and Arabidopsis transgenic systems. Molecular Biotechnology 48(1): 49-59, 2011

Transgenic rice plants produced by direct uptake of delta endotoxin protein gene from bacillus thuringiensis into rice protoplasts. Scientia Agricultura Sinica 22(6): 1-5, 1989

Transgenic rice plants produced by direct uptake of the delta -endotoxin protein gene from Bacillus thuringiensis into rice protoplasts. Rice genetics II: Proceedings of the Second International Rice Genetics Symposium, 14-18 May 1990: 605-611, 1991