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Frequency improvement of somatic embryogenesis at high embryo density by partial replacement of medium in carrot suspension cultures



Frequency improvement of somatic embryogenesis at high embryo density by partial replacement of medium in carrot suspension cultures



Journal of Fermentation & Bioengineering 84(3): 275-278



The effects of medium replacement on the development of embryos from the globular to torpedo-shaped stage were investigated in carrot suspension cultures. Partial replacement of the medium was more effective than total or non-replacement at a high embryo density. The frequency of somatic embryogenesis (embryo development from globular to torpedo-shaped) at a high embryo density (500 globular embryos/ml) was increased to 53.1 % by partial replacement of the medium as compared to 36.8% without medium replacement. Partial replacement of the medium is a simple method that should prove useful for the mass production of somatic embryos.

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Accession: 003146627

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DOI: 10.1016/s0922-338x(97)82070-3



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