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Galactose competitive inhibition of beta -galactosidase (Aspergillus oryzae) immobilized on chitosan and nylon supports

Galactose competitive inhibition of beta -galactosidase (Aspergillus oryzae) immobilized on chitosan and nylon supports

Enzyme and Microbial Technology 23(1/2): 101-106

ISSN/ISBN: 0141-0229

Inhibition of beta -galactosidase (from A. oryzae) immobilized on chitosan beads or a nylon membrane (Immunodyne) was studied. In both cases, competitive inhibition was found. The Ki value for the beta -galactosidase/chitosan system was higher than that for the beta -galactosidase/Immunodyne system, indicating that the former system was more appropriate for lactose hydrolysis.

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