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Gene cloning and sequencing of triosephosphate isomerase of Schistosoma japonicum Chinese strain (SjC-TPI)


Gene cloning and sequencing of triosephosphate isomerase of Schistosoma japonicum Chinese strain (SjC-TPI)



Chinese Journal of Schistosomiasis Control 9(6): 321-325



A pair of primers (P1 and P2) was synthesized according to the DNA sequence of Schistosoma japonicum Philippine strain. The 5'-end of primers P1 and P2 included the restriction site of BamH1 and Sal1, respectively. Messenger RNA (mRNA) of adult S. japonicum Chinese strain was prepared and a DNA fragment of the triosephosphate isomerase (TPI) gene of S. japonicum Chinese strain was amplified from the mRNA with RT-PCR.

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