Genetic transformation of gentian using wild-type Agrobacterium rhizogenes
Hosokawa, K.; Matsuki, R.; Oikawa, Y.; Yamamura, S.
Plant Cell Tissue and Organ Culture 51(2): 137-140
ISSN/ISBN: 0167-6857 DOI: 10.1023/a:1005962913491
Agrobacterium rhizogenes A4 harboring the agropine type root-inducing plasmid (pRiA4) induced hairy roots on stem sections of gentian plants. Transgenic gential plants were regenerated from the hairy roots at a frequency of 19% adventitious shoot regeneration on Murashige and Skoog medium containing 30 g l-1 sucrose, 10 mg l-1 N-phenyl-N'-1,2,3-thiadiazol-5-yl urea, 1 mg l-1 1-naphthaleneacetic acid and 2 g l-1 gellan gum. The shoots were grown to maturity in a growth chamber after acclimatization. The mature transformed plants showed distinct variations in their phenotypes, such as dwarfness. Polymerase chain reaction and Southern blot analysis revealed stable integration of transferred DNA.