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High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine



High-efficiency somatic embryogenesis and plant regeneration from suspension cultures of grapevine



Plant cell reports 18(7-8): 533-537



Embryogenic suspensions of grapevine (Vitis vinifera L.) were initiated from somatic embryos of 'Thompson Seedless' and 'Chardonnay'. Suspension cultures consisted of proembryonic masses (PEM) that proliferated without differentiation in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D). 'Chardonnay' somatic embryos developed fully from PEMs following subculture in medium without 2,4-D; however, somatic embryo development did not advance beyond the heart stage in 'Thompson Seedless' suspension cultures. Highly synchronized development of somatic embryos was obtained by inoculating < 960-micrometer PEMs into liquid medium without 2,4-D. Somatic embryos were also produced in large numbers from suspension-derived PEMs of both cultivars on semisolid medium lacking 2,4-D. Somatic embryos matured and regenerated into plants in MS basal medium containing 3% sucrose. Using this method more than 60% of the somatic embryos regenerated plants. More than 90% of the regenerated plants were successfully transferred to the greenhouse.

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Accession: 003160647

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DOI: 10.1007/s002990050617


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