An investigation was carried out to test a number of crop plants and weed species to determine the host range of pumpkin yellow vein mosaic virus (PYVMV). Adult whiteflies (Bemisia tabaci) were used to inoculate a number of common weeds (Acalypha indica, Boerhaavia diffusa, Croton bonplandianus, Euphorbia geniculata, Malvastrum coromandelianum and Lagenaria vulgaris) with PYVMV. Most of the weed species tested showed symptom development.