EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Human erythropoietin gene expression in primary cultured oviduct cells from estrogen-stimulated chicks



Human erythropoietin gene expression in primary cultured oviduct cells from estrogen-stimulated chicks



Animal Science & Technology 68(12): 1133-1136



The present study was conducted to investigate whether or not the human erythropoietin gene is expressed in primary cultured oviduct cells from estrogen-stimulated chicks. The erythropoietin gene was transfected by calcium phosphate precipitation, and erythropoietin mRNA level was determined by RNA:RNA solution hybridization during the culture periods for up to 72 hrs. The results indicated that the human erythropoietin gene was actually transcribed in the chicken oviduct cells, and erythropoietin mRNA concentration increased on the basis of both per cells and per total nucleic acids as the culture period went by, reaching maximal values at 72 hrs.

(PDF 0-2 workdays service: $29.90)

Accession: 003162834

Download citation: RISBibTeXText



Related references

Gene gun-mediated human erythropoietin gene expression in primary cultured oviduct cells from laying hens. Asian-Australasian Journal of Animal Sciences 12(1): 9-14, 1999

Gene expression changes induced by estrogen and selective estrogen receptor modulators in primary-cultured human endometrial cells: signals that distinguish the human carcinogen tamoxifen. Toxicology 206(1): 91-109, 2004

FOSP-1 (frog oviduct-specific protein-1) gene: cloning of cDNA and induction by estrogen in primary cultures of Xenopus oviduct cells. Molecular and Cellular Endocrinology 59(3): 241-248, 1988

Fosp 1 frog oviduct specific protein 1 gene cloning of complementary dna and induction by estrogen in primary cultures of xenopus oviduct cells. Molecular & Cellular Endocrinology 59(3): 241-248, 1988

Comparison of basal gene expression profiles and effects of hepatocarcinogens on gene expression in cultured primary human hepatocytes and HepG2 cells. Mutation Research 549(1-2): 79-99, 2004

Estrogen stabilization of the mRNA of chicken ovalbumin gene in the cultured organ from estrogen induced-chick oviduct. Bioscience Biotechnology & Biochemistry 58(2): 261-264, 1994

Gene expression in erythropoietin stimulated blood cells. Blood 92(10 SUPPL 1 PART 1-2): 152B, Nov 15, 1998

Estrogen-regulated expression of protein tyrosine phosphatase gamma gene in cultured human breast cells. FASEB Journal 9(3): A547, 1995

Effect of estrogen on gene expression in the chick oviduct effect of estrogen on the sequence and population complexity of chick oviduct poly adenylate containing rna. Journal of Biological Chemistry 251(12): 3738-3748, 1976

Targeted suppression of calpain-10 expression impairs insulin-stimulated glucose uptake in cultured primary human skeletal muscle cells. Molecular Genetics and Metabolism 91(4): 318-324, 2007

Role of the Rho GTPase in bradykinin-stimulated nuclear factor-kB activation and IL-1b gene expression in cultured human epithelial cells. Journal of Immunology 160(6): 38-45, 1998

Effect of bisphenol A on human chorionic gonadotrophin-stimulated gene expression of cultured mouse Leydig tumour cells. Reproductive Toxicology 24(2): 265-275, 2007

Effect of estrogen on gene expression in the chick oviduct. Effect of estrogen on the sequence and population complexity of chick oviduct poly(A)-containing RNA. Journal of Biological Chemistry 251(12): 3738-3748, 1976

Loss of ERE binding activity by estrogen receptor-alpha alters basal and estrogen-stimulated bone-related gene expression by osteoblastic cells. Journal of Cellular Biochemistry 103(3): 896-907, 2008

Loss of ERE binding activity by estrogen receptor-alpha alters basal and estrogen-stimulated bone-related gene expression by osteoblastic cells. Journal of Cellular Biochemistry 103(3): 896-907, 2007