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Human monoclonal IgG antibodies derived from a patient allergic to birch pollen as tools to study the in situ localization of the major birch pollen allergen, Bet v 1, by immunogold electron microscopy






Journal of Allergy and Clinical Immunology 101(1 Pt 1): 60-66

Human monoclonal IgG antibodies derived from a patient allergic to birch pollen as tools to study the in situ localization of the major birch pollen allergen, Bet v 1, by immunogold electron microscopy

Human monoclonal antibodies (BAB1, BAB2 and BAB4), derived from an individual allergic to pollen of white birch (Betula verrucosa) [B. pendula], were incubated with ultrathin sections of birch pollen grains and binding sites were determined by scanning electron microscopy. Purified human monoclonal antibodies bound specifically to nitrocellulose-blotted natural and recombinant Bet v 1. A p-loop-like motif consisting of GXGGXG followed by a lysine, serine and/or threonine was identified starting at amino acid 47, and the high-degree of conservation of the p-loop-containing motif was also shown by comparing the Bet v 1-derived polypeptide with the databases. The loop-core motif (GNGGPG) was conserved in all sequences of Bet v 1 isovariants and in all Bet v 1-homologous allergens submitted to GenBank. It is concluded that a possible function of the Bet v 1 protein as a nucleotide-binding protein would be in accordance with the immunolocalization of the allergen to ribosome-rich cell areas and the nucleus, and it may explain why many atopic individuals are cross-sensitized against these conserved proteins.


Accession: 003162994

PMID: 9449502

DOI: 10.1016/S0091-6749(98)70194-0



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