+ Translate
+ Most Popular
Sobre os generos Lopadusa Stal e Bothrocoris Mayr (Hemiptera, Pentatomidae, Pentatomini)
RFEF pilot plant for inactivation of Escherichia coli in apple juice
Calpionella alpina Lorenz als "Leitfossil"
Sooretama: The Atlantic Rain Forest of Brazil. Francis Dov Por
Novel approaches in myeloma therapy
Preliminary Notes on the Study of Mitochondria During the 1st Stages of the Destruction of Cadavers
A preliminary study of the biology of lichee stink bug, Tessaratoma papulosa Drury, and its control
The status of the Sabu Island python - Die Situation des Savu-Pythons
Randomised trial of intravenous immunoglobulin as prophylaxis against infection in plateau-phase multiple myeloma. the UK Group for Immunoglobulin Replacement therapy in Multiple Myeloma
Conflicts between children
Clinical presentations in neuropsychiatry
Method of suturing a perforated ulcer of the stomach and duodenum
Identification of mechanical asphyxiation in cases of attempted masking of the homicide
Effects of endogenous ascorbate on oxidation, oxygenation, and toxicokinetics of cell-free modified hemoglobin after exchange transfusion in rat and guinea pig
Is it warm? Is it real? Or just low spatial frequency?
Additions and corrections to the list of Korean Heteroptera
Factors contributing to the maintenance of oligotrophy in an alpine glacial moraine lake (Wallowa lake) in Northeast Oregon
Classification, paleoecology, and biostratigraphy of crinoids from the Stull Shale (Late Pennsylvanian) of Nebraska, Kansas, and Iowa
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Neue Formen der Bauchsammler aus Turkmenien (Hymenoptera, Apoidea)
Atypische Hühnerpest und Newcastle-Krankheit. Ein Identifizierungsversuch
Parasitellus fucorum in the debris of Apis mellifera - a risk of confusion with Tropilaelaps spp
Applying the Technology Acceptance Model to the introduction of healthcare information systems
Ein akarologischer Nachruf für den "Roland"
Mutation research on crop plants in Argentina

Interspecific somatic hybrids between wild potato Solanum acaule Bitt. and anther-derived dihaploid potato (Solanum tuberosum L.)

Interspecific somatic hybrids between wild potato Solanum acaule Bitt. and anther-derived dihaploid potato (Solanum tuberosum L.)

Plant Cell Reports 18(1/2): 82-88

ISSN/ISBN: 0721-7714

DOI: 10.1007/s002990050536

Solanum acaule Bitt. is a disomic tetraploid (4x) wild potato species which is resistant to several potato diseases. Introgression of disease resistance and abiotic stress tolerance to the tetrasomic tetraploid (4x) cultivated potato (S. tuberosum L.) gene pool via crossing has been limited due to the difference in the endosperm balance number. In the present study, protoplast fusion was applied to produce hexaploid (6x) somatic hybrids between the parental lines, tetraploid (4x) S. acaule and two anther-derived dihaploid (2x) lines of S. tuberosum cv. White Lady. One callus (0.4%) of a total of 229 calli obtained regenerated into shoots in the fusion combination S. acaule (+) White Lady 15.dh.8.2.2. All the regenerated shoots were confirmed to be interspecific somatic hybrids using species-specific RAPD markers. In another fusion combination, S. acaule (+) White Lady 7.dh.23.1.1, fifteen calli (5%) regenerated into a total of sixteen shoots from 289 calli. All the analysed somatic hybrids between S. acaule and S. tuberosum were hexaploid. The mean DNA content (2C value) of the combination S. acaule (+) White Lady 15.dh.8.2.2 somatic hybrids (4.55 pg), was approximately the sum (4.69 pg) of the DNA contents of the parental lines, S. acaule (2.95 pg) and S. tuberosum (1.74 pg). In the greenhouse, the two somatic hybrids analysed were normal in their morphological characteristics and more vigorous than their parental lines. Most of the morphological characteristics were closer to the tetraploid S. acaule than to the dihaploid S. tuberosum. The interspecific somatic hybrids are currently being tested for frost tolerance and glycoalkaloid composition.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003181639

Download citation: RISBibTeXText

Related references

Crossability of the South American wild potato species Solanum acaule Bitt. to the cultivated potato Solanum tuberosum L. Dissertation Abstracts 25(11): 6168-6169, 1965

Glycoalkaloid aglycone accumulations associated with infection by Clavibacter michiganensis ssp. sepedonicus in potato species Solanum acaule and Solanum tuberosum and their interspecific somatic hybrids. Plant Cell Reports 23(10-11): 683-691, 2005

Crossability of the South American wild potato species Solanum acaule Bitt. with the cultivated Solanum tuberosum L. Amer. Potato J 42: 259-60, 1965

Germplasm transfer from the wild tetraploid species solanum acaule bitt. to the cultivated potato solanum tuberosum l. using 2n eggs. American Potato Journal 67(11): 737-749, 1990

Diploid hybrids between allotetraploid wild potato species Solanum acaule Bitt., S. stoloniferum Schltdl. and dihaploids of S. tuberosum L. Genetika 43(8): 1065-1073, 2007

Somatic hybridization between anther-derived dihaploid lines of Solanum tuberosum L. (potato). Current plant science and biotechnology in agriculture: ) 197-198, 1988

Variation in chromosome number in somatic hybrids of dihaploid potato solanum tuberosum and solanum brevidens. Physiologia Plantarum 73(2): 22A, 1988

Inheritance of morphological characters and glycoalkaloids in potatoes of somatic hybrids between dihaploid Solanum acauleand tetraploid Solanum tuberosum. Journal of agricultural and food chemistry 47(10): 4478-4483, 1999

Somatic hybridization between anther-derived dihaploid clones of potato (Solanum tuberosum L.) and the identification of hybrid plants by isozyme analysis. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 77(1): 49-56, 1989

Selection for yield, tuber specific gravity and high 2n pollen production in potato hybrids between Solanum tuberosum L. and the wild species Solanum chacoense Bitt. Brazilian Journal of Genetics 19(3): 459-463, 1996

Fertile somatic hybrids of Solanum etuberosum dihaploid Solanum tuberosum and their backcrossing progenies Relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131(3): 323-332, 2003

Comparison of diploid and tetraploid potato families derived from solanum phureja x dihaploid solanum tuberosum hybrids and their vegetatively doubled counterparts. Euphytica 46(1): 15-34, 1990

Improved isolation of dihaploid Solanum tuberosum protoplasts and the production of somatic hybrids between dihaploid Solanum tuberosum and Solanum brevidens. In vitro cellular and developmental biology: journal of the Tissue Culture Association 23(8): 575-580, 1987

Interspecific somatic hybrids between dihaploid Solanum tuberosum L. and the wild species, S. pinnatisectum Dun. Journal of Experimental Botany 45(279): 1433-1440, 1994

Characterization of morphological variation and cold resistance in interspecific somatic hybrids between potato solanum tuberosum l. and solanum brevidens phil. Euphytica 57(1): 37-50, 1991