+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Isolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms



Isolation and mapping of two porcine skeletal muscle myosin heavy chain isoforms



Animal Genetics 29(2): 91-97



The present paper describes the isolation and linkage mapping of two isoforms of skeletal muscle myosin heavy chain in pig. Two partial cDNAs (pAZMY4 and pAZMY7), coding for the porcine myosin heavy chain-2B and -beta respectively, have been isolated from a pig skeletal muscle cDNA library. Four RFLPs were detected with the putative porcine skeletal myosin heavy chain-2B probe (pAZMY4) and one RFLP was identified with the putative myosin heavy chair -beta probe (pAZMY7). Two myosin heavy chain loci were mapped by linkage analysis performed with the five RFLPs against the PiGMaP linkage consortium ResPig database: the MYH1 locus, which identifies the fast skeletal muscle myosin heavy chain gene cluster, was located at the end of the map of porcine chromosome 12, while the MYH7 locus, which identifies the myosin heavy chain-alpha/-beta gene cluster, was assigned to the long arm of porcine chromosome 7.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003185218

Download citation: RISBibTeXText

PMID: 9699268

DOI: 10.1046/j.1365-2052.1998.00275.x


Related references

Isolation, sequencing and genetic mapping of myosin heavy-chain clones from porcine skeletal muscle. Animal Genetics 27(Suppl. 2): 77, 1996

Comparative sequence analysis of four myosin heavy chain isoforms expressed in porcine skeletal muscles: sequencing and characterization of the porcine myosin heavy chain slow isoform. Animal Science Journal 73(4): 257-262, 2002

Sequential accumulation of mRNAs encoding different myosin heavy chain isoforms during skeletal muscle development in vivo detected with a recombinant plasmid identified as coding for an adult fast myosin heavy chain from mouse skeletal muscle. Journal of Biological Chemistry 258(22): 13867-13874, 1983

Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle. Genetics and Molecular Research 13(4): 10231-10240, 2014

Identification of SNPs, mapping and analysis of allele frequencies in two candidate genes for meat production traits: the porcine myosin heavy chain 2B (MYH4) and the skeletal muscle myosin regulatory light chain 2 (HUMMLC2B). Animal Genetics 34(3): 221-225, 2003

Favourable associations between the myosin heavy-chain and light-chain isoforms in human skeletal muscle. Pflugers Archiv 416(6): 689-693, 1990

Myosin heavy chain isoforms in human skeletal muscle. European Journal of Cell Biology Suppl. (21): 34, 1987

Postnatal myosin heavy chain isoforms in prenatal porcine skeletal muscles: insights into temporal regulation. Anatomical Record. Part A Discoveries in Molecular Cellular and Evolutionary Biology 273(2): 731-740, 2003

Effect of myosin heavy chain isoforms on muscle fiber characteristics and meat quality in porcine longissimus muscle. Journal of Muscle Foods 17(4): 413-427, 2006

Changes in muscle fiber type and expression of mRNA of myosin heavy chain isoforms in porcine muscle during pre- and postnatal development. Animal Science Journal 88(2): 364-371, 2017

Functional differences of myosin heavy-chain isoforms in skeletal muscle. Naturwissenschaften. 84(5): 201-204, 1997

Three myosin heavy chain isoforms in type 2 skeletal muscle fibers. Journal of Muscle Research & Cell Motility 10(3): 197-205, 1989

Electrophoretic separation of rat skeletal muscle myosin heavy-chain isoforms. Journal of Applied Physiology 75(5): 2337-2340, 1993

Relationships between tropomyosin and myosin heavy chain isoforms in bovine skeletal muscle. Animal Science Journal 80(2): 193-197, 2010

The effect of glucocorticoids on the myosin heavy chain isoforms' turnover in skeletal muscle. Journal of Steroid Biochemistry and Molecular Biology 86(2): 201-206, 2003