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Isolation of merozoite rhoptries, identification of novel rhoptry-associated proteins from Plasmodium yoelii, P. chabaudi, P. berghei, and conserved interspecies reactivity of organelles and proteins with P. falciparum rhoptry-specific antibodies



Isolation of merozoite rhoptries, identification of novel rhoptry-associated proteins from Plasmodium yoelii, P. chabaudi, P. berghei, and conserved interspecies reactivity of organelles and proteins with P. falciparum rhoptry-specific antibodies



Experimental Parasitology 89(3): 271-284



Rhoptries were isolated from merozoites of Plasmodium yoelii (17 XL), P. chabaudi adami and P. berghei (K-173) by sucrose gradient density centrifugation. Western blotting analysis of the gradients with organelle-specific antisera revealed several major cross-reactive interspecies protein bands of approximately 235, 210, 180, 160/170, 140 and 96-110 kDa, predominantly in densities of 1.12 and 1.15 g/ml.

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Accession: 003185717

Download citation: RISBibTeXText

PMID: 9676705

DOI: 10.1006/expr.1998.4280


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