Mapping and genetic analysis of two fertility restorer loci in the wild-abortive cytoplasmic male sterility system of rice (Oryza sativa L.)

Yao, F.Y.; Xu, C.G.; Yu, S.B.; Li, J.X.; Gao, Y.J.; Li, X.H.; Zhang, Q.Fa

Euphytica 98(3): 183-187


ISSN/ISBN: 0014-2336
DOI: 10.1023/a:1003165116059
Accession: 003196277

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Hybrid varieties developed by making use of the wild abortive CMS system account for 90% of hybrid rice produced. Previous inheritance studies established that the fertility restoration in this system is controlled by 2 major loci. In this study the genomic locations of the 2 fertility restorer (Rf) loci were determined by their linkage to molecular markers. Rf containing regions were identified by surveying 2 bulk populations, composed of 30 fertile and 46 sterile plants from a large F2 population (derived from the cross Zhenshan 97A x Minghui 63), with RFLP markers covering the entire genome. The survey identified 2 likely Rf containing regions, located on chromosomes 1 and 10, respectively. This was confirmed by analysing a large random sample from the same F2 population and also with a genome-wide quantitative trait loci analysis of a testcross population. Results also showed that both loci have major effects of almost complete dominance on fertility restoration and the effect of the Rf gene on chromosome 10 is larger. The 2 loci acted as a pair of classical duplicate genes; a single dominant allele at one of the two loci would be sufficient to restore the fertility to normal or nearly normal. Closely linked markers identified in this study may be used for marker assisted selection in hybrid rice breeding programmes.