Micrografting of almond (Prunus dulcis (Miller) D.A. Webb) cv. Achak

Ghorbel, A.; Chatibi, A.; Thaminy, S.; Kchouk, M.L.

Acta Horticulturae 470: 429-433


ISSN/ISBN: 0567-7572
Accession: 003201003

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In vitro grafting of almond cv. Achak was performed on aseptic rootstocks in order to estimate the success of micrografting in relation to the mode of grafting, the scion origin and the physiological state of the grafts. Both active and dormant small shoots, buds and meristematic apices were collected from field plants. Four- to 6-week-old shoots and small apical buds (3.0-5.0 mm) formed in vitro were also used. These shoots and buds grafted on top or in a T gave a high percentage of successful grafts (82.1 and 79.2% for top and T-grafts, respectively). Contamination occurred more often in material taken from the field and necrosis was observed frequently when physiologically active meristematic apices were used. These techniques have good potential for use in the micropropagation of almond cv. Achak and represent a possible method for the renewal of almond orchards in Tunisia where trees suffer severe viral attacks.