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Molecular cloning and expression of Arabidopsis fatty acid hydroperoxide lyase



Molecular cloning and expression of Arabidopsis fatty acid hydroperoxide lyase



Plant & Cell Physiology 40(5): 477-481



Fatty acid hydroperoxide lyase (HPOL), an enzyme of the octadecanoid pathway that forms carbon-6 aldehydes such as n-hexanal or (z)-3-hexenal, was cloned from Arabidopsis thaliana as a full-length cDNA. The HPOL activity obtained by expressing the cDNA in Escherichia coli formed n-hexanal from linoleic acid 13-hydroperoxide, whereas linoleic acid 9-hydroperoxide was not a substrate for the enzyme. The HPOL mRNA is expressed at low level in leaves; however, its accumulation can be found in the inflorescence. Wounding or methyl jasmonate treatments increase the mRNA level in leaves. These results indicate that the HPOL gene is up-regulated in leaves in response to wounding and that the enzyme may be an active component of the octadecanoid defense response.

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Accession: 003205032

Download citation: RISBibTeXText

PMID: 10427771

DOI: 10.1093/oxfordjournals.pcp.a029567


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