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Postharvest changes in endogenous cytokinins and cytokinin efficacy in potato tubers in relation to bud endodormancy



Postharvest changes in endogenous cytokinins and cytokinin efficacy in potato tubers in relation to bud endodormancy



Physiologia Plantarum 103(1): 59-69



Using an indirect enzyme-linked immunosorbent assay (ELISA), the effects of postharvest storage duration and temperature on endogenous cytokinins in potato (Solanum tuberosum L. cv. Russet Burbank) tuber apical bud tissues in relation to endodormancy status were determined. Following fractionation by HPLC, a total of eight cytokinins were detected and these were: zeatin riboside-5'-monophosphate (ZRMP), zeatin-O-glucoside (ZOG), zeatin (Z), zeatin riboside (ZR), isopentenyl adenosine-5'-monophosphate (IPMP), isopentenyl adenine-9-glucoside (IP-9-G), isopentenyl adenine (IP) and isopentenyl adenosine (IPA). Regardless of postharvest storage temperature or endodormancy status, IP-9-G was the most abundant cytokinin detected while ZRMP and ZOG were the least abundant ones. In tubers preincubated at a growth-permissive temperature (20degreeC) prior to extraction, the loss of endodormancy was preceded by significant increases in the endogenous levels of Z, ZR, IPMP and IP-9-G. When stored continuously at a growth-inhibiting temperature (3degreeC), significant increases in ZR, IP-9-G and IP + IPA were observed. The total content of cytokinins increased by over 7-fold during postharvest storage and this increase was a result of de novo biosynthesis. Dose-response studies using IPA and ZR demonstrated a time-dependent increase in apparent cytokinin sensitivity during postharvest storage. With the exception of IP-9-G, injection of any of these endogenous cytokinins resulted in the rapid and complete termination of tuber endodormancy. The significance of these results with respect to endodormancy regulation and the possible mechanisms controlling cytokinin levels in potato tubers are discussed.

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Accession: 003236521

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DOI: 10.1034/j.1399-3054.1998.1030108.x


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