Section 4
Chapter 3,243

Production and characterisation of an antiserum raised against recombinant rainbow trout (Oncorhynchus mykiss) MHC class II beta-chain (MhcOnmy-DAB)

Lierop, M.J.C. van; Knight, J.; Secombes, C.J.; Hermsen, T.T.; Groeneveld, A.; Stet, R.J.M.

Fish and Shellfish Immunology 8(4): 231-243


ISSN/ISBN: 1050-4648
DOI: 10.1006/fsim.1997.0129
Accession: 003242643

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The purified product of cloning a cDNA fragment encoding most of the extracellular part of the MHC class II beta chain (MhcOnmy-DAB) into a bacterial expression vector was used to raise an antiserum in rabbits. The antiserum was specific for the recombinant Omny-DAB product and recognized a 34-kDa band in Western blots after SDS-PAGE separation of cell membrane preparations from spleen or pronephros, but not from muscle tissue.

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