EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat






Journal of General Virology 79: 95-99

Protein-binding properties of the putative AP-1 and ATF sequences in the feline immunodeficiency virus long terminal repeat

Electrophoresis-mobility-shift assays with nuclear extracts from a feline renal cell line and a T-lymphoblastoid cell line revealed that the AP-1 and ATF sites of feline immunodeficiency virus (FIV) TM2 strain had similar protein-binding properties to those of FIV Petaluma strain and consensus sequences of AP-1 and ATF sites, and that nuclear factors binding to these sites differed between the two cell lines. Cross-competition and gel-supershift assays demonstrated that the AP-1 and ATF sites had similar protein-binding properties. The effects of internal deletions of AP-1 and/or ATF sites on the basal promoter activity were also examined. Although deletion of either site moderately reduced activity, a mutant deleted in both sites had dramatically reduced activity. Therefore, we suggest that these two sites co-operatively regulate transcriptional activity of the promoter.

(PDF same-day service: $19.90)

Accession: 003245986

PMID: 9460929

DOI: 10.1099/0022-1317-79-1-95



Related references

The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat. Virus Research 51(2): 203-212, 1997

The effects of treatment with chemical agents or infection with feline viruses on protein-binding properties of the feline immunodeficiency virus long terminal repeat. Virus Research 51(2): 203-212, 1998

Sequences within the feline immunodeficiency virus long terminal repeat that regulate gene expression and respond to activation by feline herpesvirus type 1. Virology 190(1): 465-468, 1992

The AP-1 binding site in the feline immunodeficiency virus long terminal repeat is not required for virus replication in feline T lymphocytes. Journal of General Virology 74: 1573-1580, 1993

Regulatory properties of the integrated long terminal repeat of the feline immunodeficiency virus. Virus Research 41(2): 201-207, 1996

Effects of insertion of multiple AP-1 binding sites into the U3 region of the long terminal repeat of feline immunodeficiency virus. Archives of virology39(1-2): 37-48, 1994

Cis- and trans-regulation of feline immunodeficiency virus: identification of functional binding sites in the long terminal repeat. Journal of General Virology 75: 545-554, 1994

Activation of feline immunodeficiency virus long terminal repeat by feline herpesvirus type 1. Virology 184(1): 449-454, 1991

Roles of the auxiliary genes and AP-1 binding site in the long terminal repeat of feline immunodeficiency virus in the early stage of infection in cats. Journal of Virology 70(12): 8518-8526, 1996

Effects of deletions of the AP-1 and ATF sites of the feline immunodeficiency virus long terminal repeat on virus replication in primary feline peripheral blood lymphocytes. Journal of Cellular Biochemistry Supplement 0(21B): 186, 1995