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Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR)



Rapid cloning of mouse DNA as yeast artificial chromosomes by transformation-associated recombination (TAR)



Mammalian Genome 9(2): 157-159



This paper describes how the technique of cloning by transformation-associated recombination (TAR) was adapted for the rapid and efficient cloning of mouse DNA as large linear and circular yeast artificial chromosomes. Circular yeast artificial chromosomes generated with pVC-B2-B2-NeoR can be efficiently transfected to mammalian cells by the spheroplast fusion technique. It is proposed that TAR cloning with unique mouse hooks or a unique hook and a B1 or B2 element can be used to isolate genes or specific chromosome regions from the mouse genome.

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Accession: 003249899

Download citation: RISBibTeXText

PMID: 9457679

DOI: 10.1007/s003359900708


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