Restriction fragment length polymorphism and random amplified polymorphic DNA analysis of chickpea accessions

Banerjee, H.; Pai, R.A.; Sharma, R.P.

Biologia Plantarum 42(2): 197-208


ISSN/ISBN: 0006-3134
DOI: 10.1023/a:1002152416883
Accession: 003260340

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Genetic diversity analysis was carried out in 26 chickpea (Cicer arietinum) accessions using RFLP and RAPD techniques. RFLP analysis using 26 PstI sub-genomic clones on 10 chickpea accessions in 130 probe-enzyme combinations detected polymorphism with only 2 clones. PstI clone CG141 detected polymorphism in ICC4918 and Pusa209 while CG500 detected some polymorphism in Pusa261, ILC26 and in ILC13326. Analysis using 10 EcoRI clones on 12 chickpea accessions showed better hybridisation signals and one clone detected polymorphism in Pusa 256. RFLP analysis of both cultivated and wild Cicer species using heterologous DNA probe Cab3C revealed polymorphism only in wild Cicer reticulatum accession JM 2100. RAPD analysis of 13 chickpea accessions, including mutants of C235 and E100Y showed greater degree of polymorphism with 1-5 unique DNA bands for all the accessions. Phylogenetic analysis of the RAPD data helped to group the accessions. C235 and its mutants were closely grouped while E100Y and its mutant E100Ym grouped apart. Desi and kabuli chickpea accessions however, could not be separately grouped.