+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp

Ribosomal small subunit sequence variation within spores of an arbuscular mycorrhizal fungus, Scutellospora sp

Molecular Ecology 8(6): 915-921

Roots of bluebell (Hyacinthoides nonscripta) were sampled from a woodland in Yorkshire, UK and spores of an arbuscular mycorrhizal fungus Scutellospora sp., were obtained from the surrounding soil. Partial small subunit (SSU) ribosomal RNA sequences were amplified from both roots and spores using either the universal forward primer SS38 or the Glomales-specific primer VANS1, with the reverse Gigasporaceae-specific primer VAGIGA. Amplified products were cloned and sequenced. Both spores and roots yielded sequences related to those known from fungi within the Glomales, with up to four distinct SSU sequences obtained from individual spores. The VANS1 primer-binding site varied considerably in sequence and only a subset of Scutellospora sequences were amplified when the VANS1 primer was used. In addition to glomalean sequences, a number of different sequences, apparently from ascomycetes, were obtained from both root and spore samples.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003261783

Download citation: RISBibTeXText

PMID: 10434413

DOI: 10.1046/j.1365-294x.1999.00642.x

Related references

Identification and isolation of two ascomycete fungi from spores of the arbuscular mycorrhizal fungus Scutellospora castanea. Applied and Environmental Microbiology 68(9): 4567-4573, 2002

High levels of variation in ribosomal DNA sequences within and among spores of a natural population of the arbuscular mycorrhizal fungus Acaulospora colossica. Mycologia 92(2): 259-268, 2000

Characterization of a highly repeated DNA sequence (SC1) from the arbuscular mycorrhizal fungus Scutellospora castanea and its detection in planta. Applied and Environmental Microbiology 62(7): 2443-2448, 1996

Heritable variation and mechanisms of inheritance of spore shape within a population of Scutellospora pellucida, an arbuscular mycorrhizal fungus. American Journal of Botany 86(9): 1209-1216, 1999

Inter- and intrasporal nuclear ribosomal gene sequence variation within one isolate of arbuscular mycorrhizal fungus, Diversispora sp. Symbiosis 58(1-3): 135-147, 2012

Scutellospora castana, a newly described arbuscular mycorrhizal fungus. Cryptogamie Mycologie 14(4): 279-286, 1993

Scutellospora persica , an arbuscular mycorrhizal fungus new to the mycota of Poland. Mycotaxon 65: 379-390, 1997

Scutellospora hawaiiensis: A new species of arbuscular mycorrhizal fungus from Hawaii. Mycologia 87(5): 678-683, 1995

Detection of the arbuscular mycorrhizal fungus Scutellospora heterogama within roots using polyclonal antisera. Mycological Research 99(10): 1225-1232, 1995

DNA cloning and screening of a partial genomic library from an arbuscular mycorrhizal fungus, Scutellospora castanea. Mycorrhiza 4(6): 251-254, 1994

Monoxenic culture of the arbuscular mycorrhizal fungus Scutellospora calospora and Ri-TDNA transformed carrot roots. New Zealand Plant Protection 59: 97-102, 2006

Construction of genomic phage libraries of the arbuscular mycorrhizal fungi Glomus mosseae and Scutellospora castanea and isolation of ribosomal RNA genes. Mycorrhiza 6(3): 167-173, 1996

Ontogenetic comparisons of arbuscular mycorrhizal fungi Scutellospora heterogama and Scutellospora pellucida: Revision of taxonomic character concepts, species descriptions and phylogenetic hypotheses. Canadian Journal of Botany 72(1): 122-134, 1994

Interactions between an arbuscular mycorrhizal fungus (Scutellospora heterogama) and the root-knot nematode (Meloidogyne incognita) on sweet passion fruit (Passiflora alata). Brazilian Archives of Biology and Technology 53(4): 801-809, 2010

Hyphal growth from spores of the mycorrhizal fungus Glomus caldeonius: effect of amino acids Vesicular-arbuscular mycorrhizal fungi. Soil biology and biochemistry5(1): 55-58, 1983