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Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice



Semi-real time imaging of the expression of a maize polyubiquitin promoter-GFP gene in transgenic rice



Plant Science (Shannon) 124(1): 49-56



We have introduced the green fluorescent protein gene under the control of the maize ubiquitin promoter into rice embryos by microprojectile bombardment. Transient expression of GFP on the surface cells in the rice embryos depended on the localization of DNA coated gold particles. Bialaphos-resistant rice calli were produced by bombardment with a two plasmid mixture of pUbi-GFP and pDM302 carrying the coding region of the bar gene. Microscopic fluorescent image analyses indicated that the area of GFP expression increased and spread on the transgenic rice calli after heat stress.

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Accession: 003269171

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DOI: 10.1016/s0168-9452(96)04589-x


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