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Sequence analysis and expression in E. coli of segment 7 of rice dwarf virus genome



Sequence analysis and expression in E. coli of segment 7 of rice dwarf virus genome



Acta Microbiologica Sinica 36(5): 335-343



The cDNA of segment 7 (S7) of rice dwarf phytoreovirus (RDV) genome of Chinese Fujian isolate was synthesized and amplified by RT-PCR. The PCR product was cloned into pBluescript SK(+) and sequenced. The cloned segment was 1578 bp in length and contained a single open reading frame with a relative molecular weight of 56 000. Comparison of nucleotide and amino acid sequences between this segment and that of RDV genome of a Japanese isolate showed 93.2% and 94.1% homology, respectively.

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Accession: 003269723

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