+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oil

Sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oil

Molecular Breeding 4(1): 39-46

The plsC gene of Escherichia coli encoding sn-1-acylglycerol-3-phosphate acyltransferase was modified by inserting an endoplasmic reticulum retrieval signal to its 3' end and introduced into rapeseed (Brassica napus L.) plants under the control of a napin promotor. In developing seeds from transgenic plants an sn-1-acylglycerol-3-phosphate acyltransferase activity was detectable which showed substrate specificities typical of the E. coli enzyme. Moreover, seed oil from the transformants unlike that from untransformed plants contained substantial amounts of triacylglycerol species esterified with very-long-chain fatty acids at each glycerol position. Analysis of fatty acids at the sn-2 position of triacylglycerol showed hardly any very-long-chain fatty acids in untransformed plants, but in certain transformants these fatty acids were present, namely about 4% erucic acid and 9% eicosenoic acid. These data demonstrate that the bacterial acyltransferase can function in developing rapeseed and alters the stereochemical composition of transgenic rape seed oil by directing very-long-chain fatty acids, especially cis-11 eicosenoic acid, to its sn-2 position.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003273834

Download citation: RISBibTeXText

DOI: 10.1023/a:1009615229344

Related references

Expression of the Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase in plastids of transgenic plants to alter glycerolipid biosynthesis. Biological Chemistry Hoppe-Seyler 376(SPEC Suppl. ): S60, 1995

A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase mutant. European Journal Of Biochemistry. 232(3): 806-810, 1995

Lysophosphatidic acid acyltransferase from meadowfoam mediates insertion of erucic acid at the sn-2 position of triacylglycerol in transgenic rapeseed oil. Plant Physiology 109(4): 1389-1394, 1995

Lysophosphatidic Acid Acyltransferase from Meadowfoam Mediates Insertion of Erucic Acid at the sn-2 Position of Triacylglycerol in Transgenic Rapeseed Oil. Plant Physiology 109(4): 1389-1394, 1995

Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiology 120(3): 739-746, 1999

Inhibition of L-glycerol 3-phosphate acyltransferase from Escherichia coli by cis-9, 10-methylenehexadecanoic acid. Journal of Biochemistry 71(1): 99, 1972

Functional characterization of the human 1-acylglycerol-3-phosphate-O-acyltransferase isoform 10/glycerol-3-phosphate acyltransferase isoform 3. Journal of Molecular Endocrinology 42(6): 469-478, 2009

Transfer of pyro phosphate from atp and its insertion into the 3 prime position of guanosine nucleotides by a stringent factor associated with the particulate fraction from escherichia coli. Hoppe Seyler's Zeitschrift fuer Physiologische Chemie 358(12): 1613-1622, 1977

In vitro effects of chlorpromazine on glycerol-3-phosphate acyl transferase and 1-acylglycerol-3-phosphate acyltransferase in rat liver microsomes. Biochemical Pharmacology 35(22): 4083-4087, 1986

Partial purification and properties of glycerophosphate acyltransferase from rat liver. Formation of 1-acylglycerol 3-phosphate from sn-glycerol 3-phosphate and palmityl coenzyme A. European Journal of Biochemistry 31(3): 565-573, 1972

1-Acylglycerol 3-phosphate acyltransferase from rat liver. Journal of Biological Chemistry 244(13): 3677-3684, 1969

Methods for cloning 1 acylglycerol 3 phosphate acyltransferase. Fett Wissenschaft Technologie 93(11): 417-418, 1992

Approaches of cloning the 1 acylglycerol 3 phosphate acyltransferase. Biological Chemistry Hoppe-Seyler 372(8): 539, 1991

Guanosine tetraphosphate inhibition of fatty acid and phospholipid synthesis in Escherichia coli is relieved by overexpression of glycerol-3-phosphate acyltransferase (plsB). Journal of Biological Chemistry 269(42): 26584-26590, 1994

A role for 1-acylglycerol-3-phosphate-O-acyltransferase-1 in myoblast differentiation. Differentiation; Research in Biological Diversity 80(2-3): 140-146, 2011