Studies on the effects of trypsin on Newcastle disease virus La Sota strain

W.C.angXin; Zhan AiJun; Liu HuiMou; Zhang RuKuan

Journal of Yangzhou University, Natural Science Edition 2(1): 33-36


Accession: 003284783

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The La Sota strain of Newcastle disease virus (NDV) was treated with trypsin for 12 or 24 h at 37 degrees C and then used to inoculate 15-day-old chickens. The chickens survived without clinical signs. There was no significant difference in NDV haemagglutination inhibition (HI) antibody titres between experimental and control groups. The La Sota strain was then treated with trypsin for 2, 4, 12 or 24 h and used to inoculate 10-day-old chicken embryos. The HA titres were not significantly different between inoculated and control embryos. La Sota, Clone-30, V4 and F8E48 were used to inoculate chicken embryo fibroblast cell layers in DMEM agar, with or without trypsin (10 mg/litre). The non-pathogenic NDV strains were cytopathogenic only in the presence of trypsin.