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Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia

, : Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia. Journal of Biotechnology 49(1-3): 95-100

An accumulation phase for taxanes was evaluated for its effect on growth and production kinetics in Taxus brevifolia cell cultures. The second phase, tricaprylin, is an eight-carbon triglyceride that favorably partitions taxol secreted into the medium. Cell growth was generally unaffected by the presence of tricaprylin and a moderate increase in taxol synthesis over a 4-week period was observed. Adjustments of the medium pH can be used to obtain significantly higher partitioning (at higher pH), but this adversely affects the cell productivity with a net loss in taxol production for pH values greater than 7.5. Nevertheless, in situ liquid partitioning provides a clean reservoir for taxane separation in otherwise normal cell culture that can also be easily sampled and replaced during a semi-batch or continuous culture.

Accession: 003292840

DOI: 10.1016/0168-1656(96)01525-8

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Related references

Kim, J.H.; Yun, J.H.; Hwang, Y.S.; Byun, S.Y.; Kim, D.I., 1995: Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Suspension cells of Taxus brevifolia were cultured as an alternative source of taxol and related taxanes. The effects of basal medium, sugar type, and sugar concentration on growth and taxane production were investigated. To induce taxol productio...

Gibson D.; Christen A.; Ketchum R., 1991: Establishment of cell culture of taxus brevifolia for taxol and ecdysone production. Plant Physiology (Rockville) 96(1 SUPPL): 96

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Harvey S.D.; Campbell J.A.; Kelsey R.G.; Vance N.C., 1991: Separation of taxol from related taxanes in taxus brevifolia extracts by isocratic elution reversed phase microcolumn high performance liquid chromatography. Previous attempts to resolve the closely related taxanes cephalomannine and taxol by reversed-phase high-performance liquid chromatography (HPLC) on octadecyl silica columns have not been successful. In the present study, high-resolution HPLC on m...

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Strobel, G.; Stierle, A.; Hess, W.M., 1994: The stimulation of taxol production in Taxus brevifolia by various growth retardants. We have shown that chlorocholine chloride (CCC), succinic acid, 2,2-dimethylhydrazide (Alar) and tetramethylammonium bromide (TMAB) stimulate (14C)acetate incorporation into taxol in intact pieces of the inner bark of Taxus brevifolia (Pacific yew...

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