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Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia

Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia

Journal of Biotechnology 49(1-3): 95-100

An accumulation phase for taxanes was evaluated for its effect on growth and production kinetics in Taxus brevifolia cell cultures. The second phase, tricaprylin, is an eight-carbon triglyceride that favorably partitions taxol secreted into the medium. Cell growth was generally unaffected by the presence of tricaprylin and a moderate increase in taxol synthesis over a 4-week period was observed. Adjustments of the medium pH can be used to obtain significantly higher partitioning (at higher pH), but this adversely affects the cell productivity with a net loss in taxol production for pH values greater than 7.5. Nevertheless, in situ liquid partitioning provides a clean reservoir for taxane separation in otherwise normal cell culture that can also be easily sampled and replaced during a semi-batch or continuous culture.

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Accession: 003292840

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DOI: 10.1016/0168-1656(96)01525-8

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