EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia



Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia



Journal of Biotechnology 49(1-3): 95-100



An accumulation phase for taxanes was evaluated for its effect on growth and production kinetics in Taxus brevifolia cell cultures. The second phase, tricaprylin, is an eight-carbon triglyceride that favorably partitions taxol secreted into the medium. Cell growth was generally unaffected by the presence of tricaprylin and a moderate increase in taxol synthesis over a 4-week period was observed. Adjustments of the medium pH can be used to obtain significantly higher partitioning (at higher pH), but this adversely affects the cell productivity with a net loss in taxol production for pH values greater than 7.5. Nevertheless, in situ liquid partitioning provides a clean reservoir for taxane separation in otherwise normal cell culture that can also be easily sampled and replaced during a semi-batch or continuous culture.

Accession: 003292840

Download citation: RISBibTeXText

DOI: 10.1016/0168-1656(96)01525-8

Download PDF Full Text: Taxol partitioning in two-phase plant cell cultures of Taxus brevifolia



Related references

Production of taxol and related taxanes in Taxus brevifolia cell cultures: effect of sugar. Biotechnology Letters 17(1): 101-106, 1995

Establishment of cell culture of taxus brevifolia for taxol and ecdysone production. Plant Physiology (Rockville) 96(1 SUPPL): 96, 1991

Taxus spp. needles contain amounts of taxol comparable to the bark of Taxus brevifolia: analysis and isolation. Journal of Natural Products 53(5): 1249-1255, 1990

Separation of taxol from related taxanes in taxus brevifolia extracts by isocratic elution reversed phase microcolumn high performance liquid chromatography. Journal of Chromatography 587(2): 300-305, 1991

Plant antitumor agents. VI. The isolation and structure of taxol, a novel antileukemic and antitumor agent from Taxus brevifolia. Journal of the American Chemical Society 93(9): 2325-2327, 1971

Determination of taxol content in plant and cell cultures of Taxus spp. by improving HPLC method. Journal of Plant Resources and Environment 11(1): 53-54, 2002

The stimulation of taxol production in Taxus brevifolia by various growth retardants. Plant Science (Limerick) 101(2): 115-124, 1994

Taxol, an exciting anticancer drug from Taxus brevifolia: an overview. ACS symposium series: 34) 138-148, 1993

Taxol a clinically active anticancer agent from taxus brevifolia an overview. Abstracts of Papers American Chemical Society 203(1-3): AGFD111, 1992

A new large-scale process for taxol and related taxanes from Taxus brevifolia. Pharmaceutical Research 12(7): 1003-1010, 1995

Factors influencing the in vitro production of radiolabeled taxol by pacific yew taxus brevifolia. Plant Science (Shannon) 84(1): 65-74, 1992

The search for a taxol-producing microorganism among the endophytic fungi of the Pacific yew, Taxus brevifolia. Journal of Natural Products 58(9): 1315-1324, 1995

Endophytic fungi of Pacific yew (Taxus brevifolia) as a source of taxol, taxanes, and other pharmacophores. ACS symposium series: 57) 64-77, 1994

Effect of culture medium, elicitors, a plant growth regulator and a biogenetic precursor on taxol production in cell suspension cultures of Taxus cuspidata variety nana. Pakistan Journal of Biological Sciences 7(3): 399-405, 2004

Multimodal thin layer chromatographic separation of taxol and related compounds from taxus brevifolia. Journal of Liquid Chromatography 12(11): 2133-2144, 1989