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The application of molecular markers for genetic characterization of Septoria tritici populations



The application of molecular markers for genetic characterization of Septoria tritici populations



Zeitschrift fuer Pflanzenkrankheiten und Pflanzenschutz 105(5): 452-461



Molecular markers can be used for determining DNA-polymorphisms among populations of phytopathogenic fungi. We have characterized 22 single spore isolates of Septoria tritici from different locations (30 km apart from each other) in North Germany by different marker systems. On the average, restriction fragment length polymorphism (RFLP) markers cloned from single copy or repetitive DNA yielded 2.4 and 5.8 polymorphic bands, respectively. For each primer combination, random amplified polymorphic DNA (RAPD) markers gave 6.3 and amplified fragment length polymorphism (AFLP) markers 36.3 polymorphic bands, respectively. Between the groups of isolates, similarities ranged from 0.38 to 0.23 for RFLPs, 0.43 to 0.25 for AFLPs, and 0.35 to 0.15 for RAPDs, respectively. However, clustering was apparent for isolates sampled from the same location. For monitoring S. tritici infection in the field, the AFLP marker technique proved to be most powerful due to its high polymorphism rate.

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