EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

The barriers to bluetongue virus infection, dissemination and transmission in the vector, Culicoides variipennis (Diptera: Ceratopogonidae)



The barriers to bluetongue virus infection, dissemination and transmission in the vector, Culicoides variipennis (Diptera: Ceratopogonidae)



Archives of Virology 144(4): 747-761



Transmission of bluetongue virus (BTV) by a vector species of Culicoides was studied using immunohistochemistry, virus titration and in vitro transmission tests. Adult female C. variipennis were used from two colonies that are either "transmission competent" or "transmission refractory" after oral infection with BTV. Intrathoracic (i.t.) injection of BTV into the haemocoel always resulted in a fully disseminated infection and transmission of virus in saliva. However, after ingestion of an infectious blood meal, only 30% (approximately) of midges from either colony became persistently infected. Although none of the orally infected insects from the "refractory" colony were able to transmit virus, 12% of those from the "competent" colony (containing > or = 10(3.0)TCID50 of virus/midge) did transmit BTV in their saliva. The most important barriers to BTV transmission in Culicoides vector species appeared to be a mesenteron infection barrier (MIB), which controls initial establishment of persistent infection, a mesenteron escape barrier (MEB) which can restrict virus to gut cells and a dissemination barrier (DB) which can prevent virus which enters the haemocoel from infecting secondary target organs. Culicoides variipennis do not appear to present either a salivary gland infection barrier (SGIB), or a salivary gland escape barrier (SGEB) to BTV.

(PDF emailed within 0-6 h: $19.90)

Accession: 003297621

Download citation: RISBibTeXText

PMID: 10365165

DOI: 10.1007/s007050050540



Related references

Vector competence parameters of Culicoides variipennis (Diptera: Ceratopogonidae) for bluetongue virus serotype 2. Journal of Medical Entomology 30(1): 204-208, 1993

Vector competence parameters of Culicoides variipennis (Diptera: Ceratopogonidae) from bluetongue virus serotype 2. Journal of Medical Entomology. January; 301: 204-208, 1993

Vector competence parameters of Culicoides variipennis Diptera Ceratopogonidae from bluetongue virus serotype 2. Journal of Medical Entomology 30.1: 204-208, 1993

Multiplication rate of bluetongue virus in the vector Culicoides variipennis (Diptera: Ceratopogonidae) infected orally. Journal of Medical Entomology 15(3): 302-303, 1979

Infection of the midgut of Culicoides variipennis (Diptera: Ceratopogonidae) with bluetongue virus. Journal of Medical Entomology 28(1): 74-85, 1991

Microgeographic and temporal genetic variation in populations of the bluetongue virus vector Culicoides variipennis (Diptera: Ceratopogonidae). Journal of Medical Entomology 29(3): 384-394, 1992

Genetic variation in laboratory and field populations of the vector of bluetongue virus, Culicoides variipennis (Diptera: Ceratopogonidae). Journal of Medical Entomology 27(1): 24-30, 1990

Genetic differentiation among populations of Culicoides variipennis (Diptera: Ceratopogonidae), the North American vector of bluetongue virus. Annals of the Entomological Society of America, 852: 140-147, 1992

Variation in the responses of Culicoides variipennis (Diptera, Ceratopogonidae) to oral infection with bluetongue virus. Archives of Virology, 953-4: 177-182, 1987

Swarming and mating behavior of a natural population of Culicoides variipennis (Diptera: Ceratopogonidae) Biting midge, vector of bluetongue virus, ehtology, taxonomy. Journal of medical entomology, 19(2): 151-156, 1982

Bluetongue virus in sheep and cattle and Culicoides variipennis and Culicoides stellifer (Diptera: Ceratopogonidae) in Louisiana. Journal of Medical Entomology 30(4): 719-724, 1993

Absence of transovarial transmission of bluetongue virus in Culicoides variipennis: immunogold labelling of bluetongue virus antigen in developing oocytes from Culicoides variipennis (Coquillett). Comparative Biochemistry and Physiology A: 961: 19-31, 1990

Sources of variation in an elisa of bluetongue virus in culicoides variipennis diptera ceratopogonidae. Journal of Medical Entomology 28(2): 287-288, 1991

The comparative virogenesis of three serotypes of bluetongue virus in Culicoides variipennis (Diptera: Ceratopogonidae). Journal of Medical Entomology 24(1): 61-65, 1987

Detection of bluetongue virus in culicoides variipennis diptera ceratopogonidae by an antigen capture elisa. Journal of Medical Entomology 27(4): 602-606, 1990