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A cyanobacterial mutant resistant against a bleaching herbicide

Journal of Basic Microbiology 40(4): 279-288

A cyanobacterial mutant resistant against a bleaching herbicide

The nitrogen fixing cyanobacterium Nostoc muscorum has been found to be sensitive to the herbicide SAN 6706 [4-chloro-5 (dimethylamino)-2-(a,a,a,-trifluoro-m-tolyl)-3-(2H) pyridazinon (quinalphos)] at 30-45 micro M within 15 minutes. The toxicity was more severe in combined nitrogen-free (Ncomb-free) medium than in a combined nitrogen medium; this enhancement was reversible by supplementation of the medium with 3 mM glucose or 5 micro M ATP, serving as carbon and/or energy source in this organism. A mutant of this cyanobacterium resistant to 3 mM SAN 6706 was isolated and characterized to perform nitrogenase activity in exogenous ATP supplemented Ncomb-free medium. However, it exhibited moderate growth in combined nitrogen media in the absence of external ATP. The resistance factor was higher than 100. Simultaneously, this strain possesses a crossresistance to methylamine, a well-known inhibitor of photophosphorylation, irrespective of the exogenous ATP supply. The behaviour of the mutant suggests a defective phosphorylation in its photosynthetic system.

Accession: 003342483

PMID: 10986674

DOI: 10.1002/1521-4028(200008)40:4<279::AID-JOBM279>3.0.CO;2-E

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